| 黄擎擎,王丹凤,马毅,田力平.葫芦素B对人MCF-7细胞生物学行为的影响[J].济宁医学院学报,2021,44(1):10-13 |
| 葫芦素B对人MCF-7细胞生物学行为的影响 |
| The effect of cucurbitacin B on the biological behavior of MCF-7 cells |
| 投稿时间：2020-05-31 |
| DOI：10.3969/j.issn.1000-9760.2021.01.003 |
| 中文关键词: 人MCF-7细胞;葫芦素B;细胞增殖;自噬 |
| 英文关键词: MCF-7 cells;Cucurbitacin B;Cell proliferation;Autophagy |
| 基金项目: |
| 作者 | 单位 | E-mail | | 黄擎擎 | 江苏省原子医学研究所附属江原医院, 无锡 214063 | | | 王丹凤 | 江苏省原子医学研究所附属江原医院, 无锡 214063 | | | 马毅 | 江苏省原子医学研究所附属江原医院, 无锡 214063 | | | 田力平 | 苏州大学附属第一医院, 苏州 215006 | stephine01@163.com |
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| 中文摘要: |
| 目的 探讨葫芦素B对激素受体阳性的人MCF-7乳腺癌细胞生物学行为的影响及其机制。方法 培养人MCF-7细胞，随机分为对照组和20μM、50μM、100μM葫芦素B组。SRB法观测葫芦素B对人MCF-7细胞增殖活性的影响，Hoechst 33258凋亡实验观察葫芦素B对人MCF-7细胞凋亡的影响，Western Blotting检测自噬相关蛋白。结果 与对照组相比，葫芦素B各浓度组能显著抑制人MCF-7细胞增殖，增强其细胞凋亡，差异具有统计学意义（均P<0.05）；葫芦素B各浓度组LC3-Ⅱ表达水平显著升高，LC3-Ⅰ、P62表达水平显著下降（均P<0.05）。结论 葫芦素B可诱导人MCF-7细胞自噬和凋亡，抑制细胞增殖。 |
| 英文摘要: |
| Objective To investigate the effect of cucurbitacin B on the biological behavior of hormone receptor positive human MCF-7 breast cancer cells and its mechanism.Methods Human MCF-7 cells were cultured and randomly divided into control group and cucurbitacin B groups (20μM,50μM,100μM).The effect of cucurbitacin B on the proliferation of MCF-7 cells was observed by SRB method,the effect of cucurbitacin B on the apoptosis of MCF-7 cells was observed by Hoechst 33258 apoptosis assay,and autophagy related proteins were detected by Western blotting.Results Compared with the control group,cucurbitacin B could significantly inhibit the proliferation rate of human MCF-7 cells and enhance their apoptosis (all P<0.05);the expression levels of LC3-Ⅱ in cucurbitacin B groups were significantly increased,while the expression levels of LC3-I and P62 were significantly decreased (all P<0.05).Conclusion Cucurbitacin B can inhibit the proliferation of MCF-7 cells and induce autophagy and apoptosis. |
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