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| 复合造模法复制SD大鼠脂肪肝模型 |
| SD rat fatty liver model was established by compound modeling method |
| 投稿时间：2022-01-08 修订日期：2022-04-08 |
| DOI： |
| 中文关键词: 脂肪肝；大鼠；动物模型；脂肪乳剂；白酒；CCl4 |
| 英文关键词: fatty liver；rat；animal model；fat emulsion；alcohol；CCl4 |
| 基金项目:2019年度安徽高校自然科学研究重点项目(KJ2019A1270)。 |
| 作者 | 单位 | 邮编 | | 钟继昌* | 皖北卫生职业学院药学系 安徽 宿州 | 234000 | | 谢金涛 | 宿州市第一人民医院药剂科 安徽 宿州 | |
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| 中文摘要: |
| 目的 采用脂肪乳剂、30%白酒灌胃和皮下注射CCl4相结合的方法建立SD大鼠脂肪肝动物模型。方法 SD大鼠随机分为正常对照组与模型组,正常对照组普通饲料喂养,模型组喂养普通饲料并给予脂肪乳剂、30%白酒灌胃,皮下注射CCl4,连续4周。动态观察一般情况及体重变化,HE染色观察肝脏病理动态变化过程,4周末计算肝指数、检测各组大鼠生化血清指标及肝组织中TC、TG、FFA含量。结果 模型组大鼠体重显著低于正常对照组(P<0.05)、肝指数显著高于正常对照组(P<0.01)；镜下观察模型组大鼠肝脏脂变程度已达1/3,符合FL的特征；与正常对照组相比,模型组大鼠血清ALT、AST活性显著升高(P<0.001)；LDL、TC、TG含量显著升高(P<0.001),HDL显著降低(P<0.001),FFA显著升高(P<0.01)；模型组大鼠肝组织TC、TG、FFA含量显著高于正常对照组(P<0.001)。结论 通过4周脂肪乳剂、30%白酒灌胃与皮下注射CCl4相结合的复合造模方法可成功复制SD大鼠脂肪肝动物模型。 |
| 英文摘要: |
| ABSTRACT: OBJECTIVE The animal model of fatty liver (FL) in SD rats was established by fat emulsion, intragastric administration of 30% alcohol and subcutaneous injection of CCl4. METHODS SD rats were randomly divided into normal control group and model group. The normal control group was fed with ordinary feed, and the model group was fed with ordinary feed, gavage with fat emulsion and 30% alcohol, and subcutaneous injection of CCl4 for 4 weeks. The general situation and weight changes were dynamically observed. The dynamic changes of liver pathology were observed by HE staining. At the end of 4 weeks, the liver index was calculated and the biochemical serum indexes and the contents of TC, TG and FFA in liver tissue were measured. RESULTS The body weight of the model group was significantly lower than that of the normal control group (P < 0.05), and the liver index was significantly higher than that of the normal control group (P < 0.01). Microscopically, the degree of hepatic steatosis in the model group has reached 1/3, which is consistent with the characteristics of FL. Compared with the normal control group, the activities of serum ALT and AST in the model group were significantly higher (P < 0.001); the contents of LDL, TC and TG increased significantly (P < 0.001), HDL decreased significantly (P < 0.001), and FFA increased significantly (P < 0.01). The contents of TC, TG and FFA in liver tissue of model group were significantly higher than those of normal control group (P < 0.001). CONCLUSIONS The animal model of fatty liver in SD rats can be successfully established by the combination of 4-week fat emulsion, 30% alcohol gavage and subcutaneous injection of CCl4. |
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