| 李迎帅,曹飞,王春梅,杨春青,刘霞,王琴琴.食欲素在细胞氧化应激中的神经保护作用及其机制研究[J].济宁医学院学报,2022,45(5):335-338,343 |
| 食欲素在细胞氧化应激中的神经保护作用及其机制研究 |
| Research on the neuroprotective roles of Orexin in cell model of oxidative stress |
| 投稿时间：2022-07-22 |
| DOI：10.3969/j.issn.1000-9760.2022.05.006 |
| 中文关键词: 食欲素;氧化应激;HT-22细胞;过氧化氢;Nrf2 |
| 英文关键词: Orexin;Oxidative stress;HT-22 cell;Hydrogen peroxide;Nrf2 |
| 基金项目:2020年山东省大学生创新创业训练计划项目(S202010443058);2020年济宁医学院大学生创新训练计划项目(cx2020025);山东省自然科学基金项目(ZR2021QC039);山东省医药卫生科技发展计划项目(202002 081082)。 |
| 作者 | 单位 | E-mail | | 李迎帅 | 济宁医学院精神卫生学院, 济宁 272000 | | | 曹飞 | 济宁医学院精神卫生学院, 济宁 272000 | | | 王春梅 | 济宁医学院精神卫生学院, 济宁 272000 | | | 杨春青 | 济宁医学院精神卫生学院, 济宁 272000 | | | 刘霞 | 济宁医学院康复医学院, 济宁 272000 | | | 王琴琴 | 济宁医学院精神卫生学院, 济宁 272000 | qqwang@mail.jnmc.edu.cn |
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| 中文摘要: |
| 目的 探讨食欲素(Orexin)在氧化应激中的具体作用及其机制。方法 培养海马神经元细胞系HT-22细胞,随机分为对照组、H2O2(hydrogen peroxide)组、OA(Orexin A)组、OB(Orexin B)组、H2O2+OA组及H2O2+OB组,通过H2O2处理HT-22细胞建立氧化应激体外模型,采用显微镜分析方法检测各组细胞数量变化;利用实时荧光定量PCR(Quantitative Real-time PCR,qPCR)及蛋白印迹(Westernblotting)等方法探究转录因子Nrf2在HT-22细胞氧化应激模型中的表达水平及其作用。结果 与对照组相比,H2O2显著增强HT-22细胞的死亡(P<0.05);给予OA及OB处理后,与H2O2组相比,OA及OB均显著降低H2O2引起的HT-22细胞的死亡(P<0.05);与对照组相比,H2O2处理的HT-22细胞Nrf2的基因表达水平显著升高(P<0.05);与H2O2组相比,OA和OB显著增加HT-22细胞内的Nrf2的表达水平(P<0.05)。结论 食欲素可通过增强HT-22细胞内Nrf2的表达水平降低H2O2引起的HT-22细胞的神经损伤,进而发挥神经保护作用。 |
| 英文摘要: |
| Objective To explore the roles and the underlying mechanisms of orexin in oxidative stress.Methods HT-22 cells were randomly divided into control group,H2O2(hydrogen peroxide) group,OA (Orexin A) group,OB (Orexin B) group,H2O2+OA group and H2O2+OB group.The cell model of oxidative stress was constructed by treating HT-22 cells with H2O2.OA and OB were added to investigate the effects of orexin on cell number and morphology using microscopic analysis.The levels of Nrf2 in HT-22 cells were detected by qPCR (Quantitative Real-time PCR) and Western blotting.Results Compared with the control group,the cell number decreased significantly after H2O2 treatment (P<0.01);OA and OB couldrobustly decrease the death of HT-22 cells induced by H2O2(P<0.05);Compared with the control group,H2O2 led to a significant increase of the expression level of Nrf2 in HT-22 cells (P<0.05);Furthermore,compared with the H2O2 group,OA and OB obviously enhanced the expression levels of Nrf2 in HT-22 cells.Conclusion All of these results suggested that orexin in creased the levels of Nrf2 in HT-22 cells and decreased the injury of HT-22 cells induced by H2O2,leading to the neuroprotective roles in oxidative stress. |
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