| 李衍森,范明峰,乐瀚翔,孔令玉,洪丰,晁民.微载体6复合脂肪干细胞移植在肛瘘治疗中的应用[J].济宁医学院学报,2021,44(6):428-431,436 |
| 微载体6复合脂肪干细胞移植在肛瘘治疗中的应用 |
| Application of microcarrier 6 compound adipose stem cell transplantation in the treatment of anal fistula |
| 投稿时间：2021-05-26 |
| DOI：10.3969/j.issn.1000-9760.2021.06.012 |
| 中文关键词: 微载体6-脂肪干细胞;细胞分化;肛瘘;疗效 |
| 英文关键词: Microcarrier 6-Adipose stem cells;Cell differentiation;Anal fistula;Curative effect |
| 基金项目:国家自然基金项目（81170395）；山东省卫生健康委员会项目（2016WS0166） |
| 作者 | 单位 | E-mail | | 李衍森 | 济宁医学院附属医院, 济宁 272029 | | | 范明峰 | 济宁医学院附属医院, 济宁 272029 | | | 乐瀚翔 | 吉林大学第二医院, 长春 130041 | | | 孔令玉 | 济宁医学院附属医院, 济宁 272029 | | | 洪丰 | 济宁医学院附属医院, 济宁 272029 | | | 晁民 | 济宁医学院附属医院, 济宁 272029 | cmys333@sina.com, |
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| 中文摘要: |
| 目的 探讨微载体6复合脂肪干细胞(Microcarrier 6-ADSC)治疗肛瘘的疗效,为肛瘘微创化治疗探索新途径。方法 采用日本大耳兔建立兔肛瘘模型,造模成功后随机分成4组:Microcarrier 6-ADSC复合组、ADSC治疗组、手术组和对照组,每组6只,病理HE染色观察不同治疗方法的效果。结果 Microcarrier 6-ADSC复合组和ADSC治疗组的瘘管组织中有散布的横纹肌细胞和上皮组织,少量淋巴细胞聚集在微载体6周围,在ADSC治疗组中可见到脂肪细胞聚集;对照组可见肉芽组织和淋巴细胞浸润并可见大量坏死组织。与手术组相比,Microcarrier 6-ADSC复合组和ADSC治疗组的治愈时间明显缩短(P<0.05);结论 Microcarrier 6有利于ADSC的多向分化,可为ADSC的生存提供良好环境,促进瘘管的愈合。 |
| 英文摘要: |
| Objective To investigate the efficacy of microcarrier 6 combined with adipose stem cells(microcarrier 6-ADSC) in the treatment of anal fistula,and explore a new approach to minimally invasive treatment of anal fistula.Methods The Japanese big-eared rabbits were used to establish rabbit anal fistula models.After successful modeling,they were randomly divided into four groups:microcarrier 6-ADSC composite group,simple ADSC treatment group,and surgery group and the control group,six rabbits per group,and the effects of different treatment methods were observed by pathological HE staining examination.Results There were scattered striated muscle cells and epithelial tissues in the fistula tissues of the microcarrier 6-ADSC composite group and the simple ADSC treatment group,a small amount of lymphocytes gathered around the microcarrier 6,and adipose cells could be seen in the simple ADSC treatment group;the control group showed granulation tissue and lymphocyte infiltration and a large amount of necrotic tissue.Compared with the surgery group,the healing times of the microcarrier 6-ADSC composite group and the ADSC treatment group were significantly shorter(P<0.01).Conclusion Microcarrier 6 is conducive to the multidirectional differentiation of ADSCs,provides a good environment for the survival of ADSCs,and promotes the healing of fistulas. |
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