| 李超,吴磊,张艳淑.紫茎泽兰羟基泽兰酮在大鼠体内毒代动力学与组织分布[J].济宁医学院学报,2021,44(4):238-241 |
| 紫茎泽兰羟基泽兰酮在大鼠体内毒代动力学与组织分布 |
| Toxicokinetics and tissue distribution of Euptox A in rats |
| 投稿时间：2021-06-18 |
| DOI：10.3969/j.issn.1000-9760.2021.04.003 |
| 中文关键词: 紫茎泽兰羟基泽兰酮;毒代动力学;组织分布 |
| 英文关键词: Euptox A;Toxicokinetics;Tissue distribution |
| 基金项目:国家公益性行业科研专项（201103027） |
| 作者 | 单位 | E-mail | | 李超 | 华北理工大学公共卫生学院, 唐山 063210
山东省职业卫生与职业病防治研究院, 济南 250062 | | | 吴磊 | 华北理工大学公共卫生学院, 唐山 063210 | | | 张艳淑 | 华北理工大学公共卫生学院, 唐山 063210 | yanshuzhang@ncst.edu.cn |
|
| 摘要点击次数: 1172 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的 探讨紫茎泽兰羟基泽兰酮（Euptox A）在大鼠体内的毒代动力学和组织分布。方法 将15只Wistar大鼠随机分为3组，每组5只；分别静脉给予Euptox A 25、50和100mg·kg-1后，于不同时间点采集血样；另18只大鼠随机分为3组，每组6只；静脉给予Euptox A 50mg·kg-1后，分别于10、60和120min后采集心、肝、脾、肺、肾、脑、胃、肠、睾丸、肌肉和血样，采用高效液相色谱法测定血浆和组织中Euptox A的含量。运用DAS 2.0药代动力学智能分析软件计算毒代动力学参数。结果 大鼠静脉给予Euptox A 25、50和100mg·kg-1后，体内毒代动力学符合线性毒代动力学特点，半衰期分别为（27.4±6.7）、（21.6±5.4）、（18.3±5.8） min；最大血药浓度分别为（2.57±0.60）、（5.83±0.94）、（10.72±2.08） mg·L-1；AUC分别为（2039.4±835.3）、（4277.2±1194.6）、（7842.7±1077.8） mg·min-1·L-1。大鼠静脉给予Euptox A 50mg·kg-1后10min，各组织含量达到最高，60min后明显下降，在体内各组织分布广泛，其中血液、肝脏和心脏中分布较高。结论 大鼠静脉给予Euptox A后代谢符合线性毒代动力学特点，在大鼠体内分布广泛，且快速消除。 |
| 英文摘要: |
| Objective To investigate the toxicokinetics and tissue distribution of Euptox A in rats.Methods 15 Wistar rats were randomly divided into 3 groups with 5 rats in each group.Blood samples were collected at different time points after intravenous injection of Euptox A 25, 50 and 100 mg·kg-1.The other 18 rats were randomly divided into 3 groups with 6 rats in each group.After intravenous injection of Euptox A 50 mg·kg-1, heart, liver, spleen, lung, kidney, brain, stomach, intestine, testis, muscle and blood samples were collected 10, 60 and 120 minutes later.The content of Euptox A in plasma and tissue was determined by high performance liquid chromatography.The toxicokinetic parameters were calculated by DAS 2.0 intelligent pharmacokinetic analysis software.Results After intravenous injection of Euptox A 25, 50 and 100 mg·kg-1, the toxicokinetics of rats accorded with the linear toxicokinetic characteristics.The average half-life were 27.4±6.7, 21.6±5.4, 18.3±5.8 min, the maximum blood concentration were 2.57±0.60, 5.83±0.94, 10.72±2.08 mg·L-1, the area under the curve were 2039.4±835.3, 4277.2±1194.6, 7842.7±1077.8 mg·min-1·L-1, respectively.10 minutes after the intravenous injection of Euptox A at a dose of 50 mg·kg-1, the content of Euptox A reached the highest level.After 60 minutes, the content of Euptox A decreased significantly.It is widely distributed in the body and higher in the blood, liver and heart.Conclusion Intravenous administration of Euptox A in rats conformed to the characteristics of linear toxicokinetics, which was widely distributed in rats and rapidly eliminated. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
