文章摘要
高文明,李嘉铮,王慧青,董康,孟尧,程葆华.Ghrelin对6-OHDA诱导SH-SY5Y细胞线粒体损伤和细胞凋亡的影响[J].济宁医学院学报,2021,44(3):179-183,188
Ghrelin对6-OHDA诱导SH-SY5Y细胞线粒体损伤和细胞凋亡的影响
Effects of ghrelin on 6-OHDA-induced mitochondrial famage and apoptosis in SH-SY5Y cells
投稿时间:2021-03-29  
DOI:10.3969/j.issn.1000-9760.2021.03.007
中文关键词: Ghrelin;6-OHDA;帕金森病;线粒体损伤;凋亡
英文关键词: Ghrelin;6-OHDA;Parkinson's disease;Mitochondrial dysfunction;Apoptosis
基金项目:济宁医学院大学生创新训练计划项目(S201910443006)
作者单位E-mail
高文明 济宁医学院基础医学院  
李嘉铮 济宁医学院临床医学院, 济宁 272000  
王慧青 山东大学齐鲁医学院, 济南, 250014  
董康 济宁医学院临床医学院, 济宁 272000  
孟尧 济宁医学院临床医学院, 济宁 272000  
程葆华 济宁医学院基础医学院 chengbh1979@163.com 
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中文摘要:
      目的 观察Ghrelin能否保护人神经母细胞癌细胞(SH-SY5Y细胞)抵抗6-OHDA所致的线粒体损伤和细胞凋亡。方法 培养SH-SY5Y细胞,随机分为对照组、Ghrelin组、6-OHDA组和6-OHDA+Ghrelin组。通过6-OHDA处理SH-SY5Y细胞构建帕金森病(Parkinson's Disease,PD)体外模型。Ghrelin预处理后,CCK-8法观测细胞活力,乳酸脱氢酶(Lactate dehydrogenase,LDH)法观察细胞毒力,JC-1染色观察线粒体膜电位(ΔΨm),BCL-2/Bax比例检测观察细胞凋亡。结果 与对照组相比,6-OHDA组细胞活力下降,细胞毒力增加,ΔΨm以及BCL-2/Bax比例下降(均P<0.05);Ghrelin预处理后,与6-OHDA组相比,6-OHDA+Ghrelin组细胞活力上升,细胞毒力降低,ΔΨm以及Bcl-2/Bax比例增高(均P<0.05)。结论 Ghrelin预处理可以保护SH-SY5Y细胞抵抗6-OHDA所致细胞损伤,具有线粒体保护功能,减少细胞凋亡。
英文摘要:
      Objective To observe whether Ghrelin can protect SH-SY5Y cells against mitochondrial damage and apoptosis caused by 6-OHDA.Methods SH-SY5Y cells were culutured and randomly divided into control group,Ghrelin group,6-OHDA group and 6-OHDA+Ghrelin group.We utilized human neuroblastoma SH-SY5Y cells exposed to 6-OHDA as a PD model in vitro.After pretreatment with Ghrelin,cell viability was measured by CCK-8,cytotoxicity was observed by LDH,and mitochondrial membrane potential (ΔΨm) by JC-1 staining.Additionally,the ratio of Bcl-2/Bax reflected the cell apoptosis.Results Compared with the control group,cell viability,ΔΨm,and the ratio of Bcl-2/Bax were decreased while the cytotoxicity was increased in the 6-OHDA group.(all P<0.05).Compared with the 6-OHDA group,pretreatment with Ghrelin increased cell vitality,ΔΨm,and the ratio of Bcl-2/Bax but decreased the cytotoxicity (all P<0.05).Conclusion Pretreatment with Ghrelin protected SH-SY5Y cells against cell damage,ameliorated the mitochondrial dysfunction,and reduced cell apoptosis induced by 6-OHDA.
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