| 李丽,高波,刘梅芳.侧柏叶对家兔心肌缺血-再灌注损伤的保护效应及其胆碱能抗炎作用[J].济宁医学院学报,2021,44(1):14-17 |
| 侧柏叶对家兔心肌缺血-再灌注损伤的保护效应及其胆碱能抗炎作用 |
| Study on the protective action of cacumen biotae on rabbits' myocardial ischemia reperfusion injury and its cholinergic anti-inflammatory function |
| 投稿时间：2020-07-09 |
| DOI：10.3969/j.issn.1000-9760.2021.01.004 |
| 中文关键词: 侧柏叶;心肌缺血-再灌注损伤;家兔;胆碱能抗炎通路 |
| 英文关键词: CB;MIRI;Rabbit;Cholinergic anti-inflammatory pathway |
| 基金项目:济宁医学院青年基金项目（JY2016KJ0082） |
| 作者 | 单位 | | 李丽 | 济宁医学院药学院, 日照 276826 | | 高波 | 济宁医学院药学院, 日照 276826 | | 刘梅芳 | 济宁医学院药学院, 日照 276826 |
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| 中文摘要: |
| 目的 探索侧柏叶（Cacumen Biotae，CB）对家兔心肌缺血-再灌注损伤（myocardial ischemia reperfusion injury，MIRI）的保护效应及胆碱能抗炎通路在此过程中的作用。方法 将家兔随机分为假手术组、模型对照组、侧柏叶乙酸乙酯提取物组以及侧柏叶迷走神经切断组，每组8只，以心率、收缩压、血清肌酸激酶（creatine kinase，CK）、乳酸脱氢酶（lactic dehydrogenase，LDH）、肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）、白细胞介素-6（interleukin-6，IL-6）、乙酰胆碱（acetyl choline，ACH）以及心肌组织总一氧化氮合成酶（nitric oxide synthetase，NOS）为检测指标，探讨CB对家兔MIRI的保护效应以及胆碱能抗炎通路在此过程中的作用。结果 模型对照组家兔心率明显减慢、收缩压明显降低，血清心肌酶CK、LDH、血清TNF-α、IL-6和Ach水平明显升高（P<0.01），心肌组织NOS含量明显降低（P<0.01）。与模型对照组相比，侧柏叶乙酸乙酯提取物组能明显减小家兔MIRI心率和收缩压的降低率（P<0.01），显著减弱血清心肌酶CK和LDH的升高（P<0.01），明显降低血清TNF-α和IL-6水平（P<0.01），增加血清Ach及心肌组织NOS含量（P<0.01），而迷走神经切断后，侧柏叶的以上作用明显减弱（P<0.01）。结论 侧柏叶对家兔MIRI具有保护作用，此作用与胆碱能抗炎通路有关。 |
| 英文摘要: |
| Objective To study the protective action of Cacumen Biotae (CB) on rabbits' myocardial ischemia reperfusion injury and the cholinergic anti-inflammatory pathway's function during this process.Methods Rabbits were divided into sham operation group,model control group,ethyl acetate extract group and CB plus vagus nerve cut group.Each group has 8 rabbits.Heart rate,blood pressure,blood levels of creatine kinase(CK),lactic dehydrogenase (LDH),tumor necrosis factor-α(TNF-α),interleukin(IL-6),acetyl choline(ACH) and the content of nitric oxide synthetase(NOS) in cardiac muscle were detected.According to these indexes,the protective action of CB on myocardial ischemia reperfusion injury and the cholinergic anti-inflammatory pathway's function during this process were evaluated.Results For the rabbits in model control group,the blood pressure and heart rate reduced,The blood level of CK,LDH,TNF-α,IL-6 and ACH rose,the content of NOS in cardiac muscle decreased (P<0.01).For the rabbits in ethyl acetate extract group,the reduction ratio of heart rate and blood pressure after myocardial ischemia reperfusion injury minished obviously (P<0.01),the rise of blood level of CK and LDH weakened evidently (P<0.01),the blood level of TNF-α,IL-6 reduced and the blood level of ACH and the content of NOS in cardiac muscle increased markedly (P<0.01).After cutting the vagus nerve,these above functions of ethyl acetate extract of CB were weakened remarkablely (P<0.01).Conclusion CB has protective function on rabbits' myocardial ischemia reperfusion injury,and this function is closely related to the cholinergic anti-inflammatory pathway. |
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