| 李晓,高慧婕,朱桐林,张腾元,王清华,刘超.阿胶对环磷酰胺所致免疫抑制小鼠的免疫保护作用[J].济宁医学院学报,2019,42(6):385-389 |
| 阿胶对环磷酰胺所致免疫抑制小鼠的免疫保护作用 |
| Immunoprotective of Colla Corii Asini on immune function in immunosuppressed mice induced by cyclophosphamide |
| 投稿时间：2018-08-21 |
| DOI：10.3969/j.issn.1000-9760.2019.06.002 |
| 中文关键词: 阿胶;环磷酰胺;肿瘤坏死因子α;粒细胞-巨噬细胞集落刺激因子 |
| 英文关键词: Colla Corii Asini;Cyclophosphamide(Cy);TNF-α;GM-CSF |
| 基金项目:山东省自然科学基金项目（ZR2016HL22）；济宁医学院大学生科研项目（JYXS2017KJ022）；济宁医学院青年教师科研扶持基金（JY2017KJ046） |
| 作者 | 单位 | E-mail | | 李晓 | 济宁医学院药学院, 日照 276826 | | | 高慧婕 | 济宁医学院药学院, 日照 276826 | | | 朱桐林 | 济宁医学院药学院, 日照 276826 | | | 张腾元 | 济宁医学院药学院, 日照 276826 | | | 王清华 | 济宁医学院药学院, 日照 276826 | | | 刘超 | 济宁医学院药学院, 日照 276826 | lcanatomy@163.com |
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| 中文摘要: |
| 目的 探究不同品牌阿胶对环磷酰胺（Cy）所致免疫功能低下小鼠的免疫调节作用。方法 ICR小鼠随机分成5组：对照组、Cy组、A品牌阿胶组、B品牌阿胶组和C品牌阿胶组。Cy腹腔注射致免疫抑制，3组阿胶组小鼠均灌服5%不同品牌阿胶溶液，7d后，测定并比较各组小鼠的脾脏和胸腺指数、小鼠血清溶血素的含量，ELISA检测小鼠血清肿瘤坏死因子α（TNF-α）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）的表达，RT-PCR检测各组小鼠脾脏TNF-α和GM-CSF mRNA的表达。结果 与Cy组相比，A、B、C 3种品牌阿胶小鼠的胸腺和脾脏指数均显著升高，小鼠血清抗体的含量显著提高，其中A、B品牌阿胶小鼠提高更为显著（P<0.001）；与Cy组相比，A、B、C 3种品牌的阿胶小鼠血清中TNF-α和GM-CSF的表达显著增加，小鼠脾脏TNF-α和GM-CSF mRNA的表达升高，其中A品牌阿胶小鼠提高更为明显（P<0.001）。结论 3种品牌阿胶对Cy导致的免疫功能低下小鼠均有免疫保护作用，提高尤以A品牌阿胶效果最好。 |
| 英文摘要: |
| Objective To explore the immunoregulatory function of different brands of Colla Corii Asini in immunosuppressed mice induced by cyclophosphamide.Methods ICR mice were randomly divided into five groups:control group,cyclophosphamide (Cy) group,and brand A,B and C Colla Corii Asini group.The immunosuppression was induced by receiving intraperitoneal injection of Cy.The mice in Colla Corii Asini group received intragastric administration of 5% brand A,B and C Colla Corii Asini solution every day.After 7 days,the thymus and spleen index,the content of serum hemolysin were analyzed.The expression of tumor necrosis factor α (TNF-α) and the granulocyte-macrophage colony-stimulating factors (GM-CSF) in peripheral blood was detected by ELISA.RT-PCR was used to detect the changes of TNF-α and GM-CSF mRNA expression in spleen cells of mouse.Results Compared with the Cy group,three brands of Colla Corii Asini increased the thoracic (spleen) index,the serum hemolysin level,while Brand A and B increased them more significantly (P<0.001).Compared with the Cy group,three brands of Colla Corii Asini increased the expression of TNF-α and GM-CSF in peripheral blood and spleen cells of mouse,while Brand A increased them more significantly (P<0.001).Conclusion Three brands of Colla Corii Asini can improve the immune function in immunodepressed mice induced by Cy.Among them,brand A has the best effect. |
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