| 张璐琳,刘文琦,蔡荣荣,沈皖珠,崔亚男,管华.血清对Hela细胞整合素αvβ3受体内吞循环的影响[J].济宁医学院学报,2019,42(3):162-165 |
| 血清对Hela细胞整合素αvβ3受体内吞循环的影响 |
| The impact of serum on the endocytic recycling of αvβ3 integrin in Hela cells |
| 投稿时间：2017-11-09 |
| DOI：10.3969/j.issn.1000-9760.2019.03.003 |
| 中文关键词: 整合素αvβ3受体;细胞内吞;血清;人宫颈癌细胞;酶联免疫吸附法 |
| 英文关键词: Integrin αvβ3;Cellular uptake;Serum;Hela cells;Enzyme linked immunosorbent assay |
| 基金项目:济宁医学院大学生创新训练计划项目（cx2016002）；国家级大学生创新训练项目（201810443046）；山东省自然科学基金青年基金（ZR2017QH006） |
| 作者 | 单位 | E-mail | | 张璐琳 | 济宁医学院药学院, 日照 276826 | | | 刘文琦 | 济宁医学院药学院, 日照 276826 | | | 蔡荣荣 | 济宁医学院药学院, 日照 276826 | | | 沈皖珠 | 济宁医学院药学院, 日照 276826 | | | 崔亚男 | 济宁医学院药学院, 日照 276826 | guancyn@sina.com | | 管华 | 济宁医学院药学院, 日照 276826 | jl790217@163.com |
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| 中文摘要: |
| 目的 探讨血清对Hela细胞整合素αvβ3受体内吞循环的影响。方法 培养Hela细胞，激光共聚焦显微镜观测其表面αvβ3受体的表达情况。通过对细胞表面蛋白进行生物素标记，利用酶联免疫吸附法，考察含血清培养基培养条件下和不含血清的培养条件下，细胞表面αvβ3受体的内吞循环情况。结果 αvβ3受体在Hela细胞表面具有高表达。与不含血清培养条件相比，含血清培养组αvβ3受体的入胞量在5min和10min时显著减少，差异具有统计学意义（P<0.05）。结论 含血清培养基培养的条件下，Hela细胞中的αvβ3受体可能更快地循环返回至细胞表面。 |
| 英文摘要: |
| Objective To determine the impact of serum on the endocytic recycling of αvβ3 integrin in Hela cells.Methods Hela cells were purchased as the cell model,and the expression of αvβ3 integrin was studied by using confocal laser scanning microscopy analysis.Capture-enzyme linked immunosorbent assay was used to investigate the endocytic recycling of αvβ3 integrins which were labelled by biotin.Results Obvious difference of the intracellular quantity of αvβ3 integrins was observed under the two culture conditions.The difference was enlarged by the end of 5min and 10min respectively.Conclusion Integrin αvβ3 would recycle back to the surface of Hela cells more quickly when the cells were cultured in complete DMEM. |
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