| 陈俊,周佳彬,李根华,秦咸蕴,靳峰.柯里拉京对神经胶质瘤U373MG细胞增殖和迁移能力的影响[J].济宁医学院学报,2019,42(3):158-161 |
| 柯里拉京对神经胶质瘤U373MG细胞增殖和迁移能力的影响 |
| Effect of Corilagin on cell proliferation and migration ability of glioma U373MG cells |
| 投稿时间：2018-10-13 |
| DOI：10.3969/j.issn.1000-9760.2019.03.002 |
| 中文关键词: 柯里拉京;U373MG;胶质瘤细胞;细胞增殖;迁移 |
| 英文关键词: Corilagin;U373MG;Glioma cells;Cell proliferation;Migration |
| 基金项目:山东省自然科学基金（ZR2016HL34）；山东省高等学校科技计划项目（J16LL05） |
| 作者 | 单位 | E-mail | | 陈俊 | 济宁医学院临床医学院, 济宁 272013
济宁医学院附属医院, 济宁 272029 | | | 周佳彬 | 济宁医学院附属医院, 济宁 272029 | | | 李根华 | 济宁医学院附属医院, 济宁 272029 | | | 秦咸蕴 | 济宁医学院附属医院, 济宁 272029 | | | 靳峰 | 济宁医学院附属医院, 济宁 272029 | jinfengsdjn@163.com |
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| 中文摘要: |
| 目的 探究柯里拉京（Corilagin）对人脑胶质瘤U373MG细胞增殖活性和迁移能力的影响。方法 培养U373MG细胞，分别应用不同浓度的Corilagin（25、50、100、200μmol·L-1）干预U373MG细胞，培养不同时间（24、48、72h）后收集细胞，Cell Counting Kit-8检测药物干预后细胞增殖活性的变化，细胞划痕实验检测200μmol·L-1 Corilagin对U373MG细胞迁移能力的影响。结果 随着Corilagin浓度的升高以及培养时间的延长，U373 MG细胞的生长密度和体积明显降低，胞体出现皱缩，并可见大量散在的细胞碎片，细胞的生长受到明显抑制；与对照组相比，U373MG细胞的存活率随着Corilagin药物浓度的升高和培养时间的延长而明显降低，差异具有统计学意义（P<0.05），在含200μmol·L-1 Corilagin的培养液培养24、48和72h后U373MG细胞迁移度均明显降低，差异具有统计学意义（P<0.05）。结论 Corilagin能有效抑制U373MG细胞的增殖活性和迁移能力。 |
| 英文摘要: |
| Objective To explore the effect of Corilagin on proliferation and migration of glioblastoma U373MG cells.Methods Glioblastoma U373MG cells were cultured.The cells were intervened by different Corilagin concentrations(25,50,100,200μmol·L-1) at different time(24,48,72h),and then collected to detect the change of cell proliferation with Cell Counting Kit-8.The effect of 200μmol·L-1 Corilagin on the migration ability of U373MG cells was examined by cell scratch assay.Results With the increase of Corilagin concentration and the prolongation of culture time,compared with the control group,the growth density and volume of U373 MG cells were significantly reduced.Meanwhile,the cell bodies shrunk,a large number of scattered cell debris were observed,and the cell growth was significantly inhibited.Compared with the control group,the survival rate of U373MG cells was decreased significantly with the increase of Corilagin drug concentration and the prolongation of culture time(P<0.05).Compared with the control group,the migration of U373MG cells was significantly decreased after 24,48 and 72h of culture with 200μmol·L-1 Corilagin(P<0.05).Conclusion Corilagin can effectively inhibit the proliferation and migration of U373MG cells. |
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