文章摘要
刘元元,李晓哲,董海新.不同时期艰难梭菌感染状况分析[J].济宁医学院学报,2018,41(6):410-412
不同时期艰难梭菌感染状况分析
Analysis of Clostridium difficile infection in different periods
投稿时间:2018-10-07  
DOI:10.3969/j.issn.1000-9760.2018.06.009
中文关键词: 艰难梭菌;毒素A/B;腹泻
英文关键词: Clostridium difficile;Diarrhea;Toxin A/B
基金项目:济宁医学院附属医院"苗圃"科研计划项目(JYFY-MP-2013-MS-010)
作者单位
刘元元 济宁医学院附属医院, 济宁 272000 
李晓哲 济宁医学院附属医院, 济宁 272000 
董海新 济宁医学院附属医院, 济宁 272000 
摘要点击次数: 640
全文下载次数: 261
中文摘要:
      目的 掌握医院艰难梭菌近几年的感染状况,为医院感染监测和防控提供现实依据。方法 收集院内2014年1月至2014年12月106例住院腹泻患者粪便标本为前期组,同时选择2016年1月至2016年12月90例住院腹泻患者粪便标本为后期组,采用艰难梭菌显色平板筛选可疑菌落,通过镜检和质谱仪进行鉴定;同时对粪便标本通过酶免疫分析方法检测毒素A、B;对于培养阳性的菌株采用PCR扩增毒素A、B基因片段;比较两个时期艰难梭菌感染情况以及不同方法检出率的差异。结果 两个检测方法厌氧培养及酶免疫分析监测艰难梭菌感染阳性率无统计学差异。同一时期不同方法阳性率比较无统计学意义,另外PCR扩增结果除后期组2株阴性外均产毒素A、B。结论 两个时期艰难梭菌感染率均较高,说明我院艰难梭菌感染形势严峻,应高度关注,及时监测,防止传播和流行。
英文摘要:
      Objective To understand the infection status of Clostridium difficile in recent years,and provide a practical basis for nosocomial surveillance,prevention and control.Methods The collected 106 fecal specimens of hospitalized patients with diarrhea from January 2014 to December 2014 was the former group,and 90 fecal specimens of hospitalized patients with diarrhea from January 2016 to December 2016 was the latter group.And the suspicious colonies were screened in Clostridium difficile color plate and identified by microscopy and mass spectrometry.Toxins A and B in fecal specimens were detected by enzyme immunoassay assay.The gene fragments of toxin A and toxin B were amplified by PCR for cultured positive strains.The infection status of clostridium difficile between two periods and the detection rates of different methods were compared.Results There were no significant differences of the positive rates with anaerobic culture and enzyme immunoassay and the different methods at the same time.Besides,except two strains of the later group toxins A and B were positive by PCR amplification.Conclusion The positive rates of clostridium difficile with different methods have no obvious differences.The infection rates of clostridium difficile during two periods are relatively higher,and the detection rates are essentially in agreement.This indicates that the situation of clostridium difficile infection is severe in our hospital,which should be paid close attention and monitored in time to prevent its spread and epidemic.
查看全文   查看/发表评论  下载PDF阅读器

分享按钮

漂浮通知

  X

近期,网上出现以《济宁医学院学报》杂志社的名义制造的虚假网站,诱骗作者在线投稿,骗取作者版面费或收取中介费,造成恶劣影响。在此提醒广大作者,审慎辨别,谨防上当。 投稿警示:我刊对所有来稿均不收取审稿费。 投稿方式:登录济宁医学院官方网站,在左下方点击“济宁医学院学报”进入《济宁医学院学报》网页,点击作者登录,注册新用户,然后在线投稿。

-->