文章摘要
张跃,翟珂,李蕊,王鸿.DCC与HER-2 mRNA在人胃癌组织的表达[J].济宁医学院学报,2018,41(5):322-325
DCC与HER-2 mRNA在人胃癌组织的表达
The preliminary study of the expression level of DCC mRNA and HER-2 mRNA in human gastric cancer tissue
投稿时间:2018-04-10  
DOI:10.3969/j.issn.1000-9760.2018.05.004
中文关键词: 胃癌;结直肠癌缺失基因;人表皮生长因子受体2;逆转录-聚合酶链反应
英文关键词: Gastric cancer;DCC;HER-2;RT-PCR
基金项目:济宁市科技计划(医药卫生)项目(2016-56-50)
作者单位
张跃 济宁市兖州区人民医院, 济宁医学院附属医院兖州院区, 济宁 272100 
翟珂 济宁市兖州区人民医院, 济宁医学院附属医院兖州院区, 济宁 272100 
李蕊 济宁市兖州区人民医院, 济宁医学院附属医院兖州院区, 济宁 272100 
王鸿 济宁市兖州区人民医院, 济宁医学院附属医院兖州院区, 济宁 272100 
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中文摘要:
      目的 探讨胃癌组织中DCC及HER-2 mRNA的转录水平与胃癌发生、发展及临床病理特征的关系。方法 采集52例胃癌患者的胃癌组织及癌旁正常组织,应用RT-PCR的方法检测其中DCC mRNA与HER-2 mRNA的转录水平,并分析其与胃癌发生、发展、转移的关系。结果 胃癌组织DCC mRNA、HER-2 mRNA检出率为84.6%、28.84%。淋巴结转移阳性患者的DCC mRNA转录水平明显低于淋巴结转移阴性者;DCC mRNA在Ⅲ期、Ⅳ期患者的转录水平明显低于I期、Ⅱ期者;高分化及中分化胃癌患者DCC mRNA转录水平明显高于低分化(未分化)者(P<0.05)。淋巴结转移阳性患者的HER-2 mRNA转录水平明显高于淋巴结转移阴性者;HER-2 mRNA在癌组织有浆膜浸润患者的转录水平明显高于无浸润浆膜者(P<0.05)。结论 胃癌的浸润和转移与HER-2和DCC的表达密切相关。DCC及HER-2基因联合检测对胃癌的基因治疗及预后提供重要依据。
英文摘要:
      Objective To detect the transcriptional level of DCC mRNA and HER-2 mRNA in gastric cancer tissue and investigate its relation with the clinical pathological features.Methods RT-PCR method was used to detect the transcriptional level of DCC mRNA and HER-2 mRNA in 52 cases of human gastric carcinoma tissues and surrounding non-tumor gastric tissues.And the transcriptional levels of DCC mRNA and HER-2 mRNA was analyzed with gastric cancer occurrence,development and metastasis.Results The expression rate of DCC mRNA and HER-2 mRNA were 84.6% and 28.84% in gastric cancer tissues respectively.The transcriptional level of DCC mRNA in patients of lymph node positive metastasis was significantly lower than that of lymph node negative metastasis.The transcriptional level of DCC mRNA in stage Ⅲ~Ⅳ patients was significantly lower than patients with stage Ⅰ~Ⅱ.The transcriptional level of DCC mRNA in patients of high-differentiated and middle-differentiated gastric carcinoma was significantly higher than that of poor-differentiated (non-differentiated) group (P<0.05).However,it was significant difference that the transcriptional level of HER-2 mRNA in patients of different groups in lymph node metastasis status and the degree of invasion,with statistical significance (P<0.05).Conclusion The invasion and metastasis of gastric cancer are closely related to the expression of HER-2 and DCC.DCC and HER-2 gene combined detection are useful prognostic factors in patients with gastric carcinoma.
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