文章摘要
孙芳,王红红,张姣,郇通,李艳.NSD2及H3K36甲基化在小鼠胚胎干细胞向神经细胞分化中的表达[J].济宁医学院学报,2018,41(3):158-162
NSD2及H3K36甲基化在小鼠胚胎干细胞向神经细胞分化中的表达
The expression of NSD2 and H3K36 methylation during the differentiation of stem cells towards neural cells
投稿时间:2018-03-22  
DOI:10.3969/j.issn.1000-9760.2018.03.002
中文关键词: NSD2;H3K36甲基化;组蛋白甲基转移酶;胚胎干细胞;神经细胞
英文关键词: NSD2;H3K36 methylation;Histone methyltransferase;Embryonic stem cells;Neural cells
基金项目:国家自然科学基金地方项目(31260270)
作者单位E-mail
孙芳 济宁医学院基础医学院, 济宁 272067  
王红红 大理大学基础医学院, 大理 671000  
张姣 大理大学基础医学院, 大理 671000  
郇通 杭州市第一人民医院, 杭州 310006  
李艳 大理大学基础医学院, 大理 671000 bigbigsmile04@aliyun.com 
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中文摘要:
      目的 在体外建立小鼠胚胎干细胞(mESCs)向神经细胞(NCs)方向分化体系,观察组蛋白甲基化转移酶NSD2及其相关的组蛋白修饰H3K36二甲基化(H3K36me2)、H3K36三甲基化(H3K36me3)在NCs发育过程中的表达变化。方法 用维甲酸(RA)诱导mESCs向NCs方向分化,通过观察细胞形态和RT-qPCR检测分子标志物来验证mESCs诱导分化效果,用Western Blot方法检测NSD2以及其相关的组蛋白甲基化修饰在诱导分化过程中的蛋白水平变化。结果 与mESCs状态相比,在mESCs向NCs方向分化的过程中,NSD2蛋白表现出先升高后下降的变化,差异具有统计学意义(均P<0.05),而其相关的组蛋白甲基化标记H3K36me2和H3K36me3蛋白表达总体变化不大,差异无统计学意义(P>0.05)。结论 ESCs向NCs分化过程中NSD蛋白表达量的改变与相关组蛋白修饰变化趋势不完全一致,组蛋白甲基化修饰总量的变化比较小,这可能是因为同时受到其他甲基化转移酶与去甲基化酶综合作用的结果。
英文摘要:
      Objective To establish an in vitro system of differentiating mouse embryonic stem cells (mESCs) into neural cells(NCs) and observe the change of the level of histone methyltransferase NSD2 and its related histone modification H3K36 di-methylation (H3K36me2) and H3K36 tri-methylation (H3K36me3) in the development of the neural cells.Methods mESCs were induced to differenciate towards NCs in vitro by retinoic acid (RA).The efficiency of mESCs differentiation was verified by cell morphology and real-time quantitative RT-RCR test of related molecular markers.The level of NSD2 protein and its related histone methylation modifications were examined during the process of differentiation by western blot.Results We observed that the level of NSD2 protein showed a sharp increase and then decreased,and the change was statistically significant (P<0.05).While no significant change was seen in its related histone methylation modifications such as H3K36me2 and H3K36me3 (P>0.05).Conclusion We observed that the change of NSD2 protein was not in conformity with its related histone methylation markers in the differentiation of mESCs towards NCs,and the histone methylation markers changed very little that may be affected by other histone methyltransferases or demethylases.
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