文章摘要
王媛媛,张雪芹,袁菲菲,余培凡,吴飘扬,田宇.川牛膝和怀牛膝多糖抗炎免疫调节活性研究[J].济宁医学院学报,2018,41(2):132-134
川牛膝和怀牛膝多糖抗炎免疫调节活性研究
Research on the anti-inflammatory and immunodulatory activity of Cyathulae Radix and Achyranthis Bidentatae Radixpolysaccharides
投稿时间:2017-09-11  
DOI:10.3969/j.issn.1000-9760.2018.02.012
中文关键词: 川牛膝多糖;怀牛膝多糖;抗炎;免疫调节;活性
英文关键词: Cyathulae Radix polysaccharide;Achyranthis Bidentatae Radix polysaccharide;Anti-inflammatory;Immunodulatory;Activity
基金项目:安徽省高校自然科学研究项目(KJ2018A0230);安徽省大学生创新创业训练计划项目(201610367038);蚌埠医学院自然科学研究项目(BYKY1658/1662)
作者单位
王媛媛 蚌埠医学院公共基础学院, 蚌埠 233030 
张雪芹 蚌埠医学院公共基础学院, 蚌埠 233030 
袁菲菲 蚌埠医学院公共基础学院, 蚌埠 233030 
余培凡 蚌埠医学院公共基础学院, 蚌埠 233030 
吴飘扬 蚌埠医学院公共基础学院, 蚌埠 233030 
田宇 蚌埠医学院公共基础学院, 蚌埠 233030 
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中文摘要:
      目的 检测川牛膝多糖和怀牛膝多糖对小鼠腹腔巨噬细胞(Ana-1细胞)中PGE2、TNF-α、IL-12因子分泌的影响,探究两种多糖的抗炎、免疫调节活性。方法 配制3.125、6.25、12.5、25、50、100、200、400μg/ml系列浓度的两种多糖溶液,体外刺激Ana-1细胞,采用ELISA技术,450nm波长处检测并计算两种多糖抑制Ana-1细胞中PGE2产生的IC50及促进TNF-α、IL-12分泌的EC50值。结果 怀牛膝多糖抑制Ana-1细胞中PGE2产生的IC50值为(46.151±1.408)μg/ml,而川牛膝多糖抑制Ana-1细胞中PGE2产生的能力较弱,并未计算出IC50值;两种多糖促进Ana-1细胞中TNF-α因子分泌的EC50值分别为(21.247±3.521)、(23.532±1.160)μg/ml;川牛膝和怀牛膝多糖促进Ana-1细胞中IL-12因子分泌的能力不明显,均未算出EC50值。结论 怀牛膝多糖在抑制Ana-1细胞中PGE2产生的模型下具有一定的抗炎活性优势;两种多糖主要通过促进Ana-1细胞中TNF-α因子分泌的途径产生免疫活性,但在此模型下两种多糖的免疫调节活性无显著性差异。
英文摘要:
      Objective To investigate the anti-inflammatory and immunodulatory activities of Cyathulae Radix polysaccharide and Achyranthis Bidentatae Radix polysaccharide by detecting their effects on PGE2,TNF-α and IL-12 secretions of Ana-1 cells in mice.Methods Concentrations of 3.125,6.25,12.5,25,50,100,200,400μg/ml of two polysaccharides were formulated to stimulate the Ana-1 cells in vitro.The IC50 inhibiting the secretion of PGE2and the EC50promoting the secretion of TNF-α,IL-12 by two polysaccharides in Ana-1cells were detectedby ELISA on the wavelength of 450nm.Results The IC50 inhibiting the secretion of PGE2 in Ana-1cells byAchyranthis Bidentatae Radix polysaccharid was (46.151±1.40)μg/ml,while that of Cyathulae Radix polysaccharide couldn't be calculated because its capacity was weak.The EC50 promoting the secretion of TNF-α by Cyathulae Radix polysaccharide and Achyranthis Bidentatae Radix polysaccharide were (21.247±3.521) μg/ml and (23.532±1.160)μg/ml,respectively.The EC50 promoting the secretion of IL-12 of two polysaccharides couldn't be calculated due to their relatively weak capacity.Conclusion Achyranthis Bidentatae Radix polysaccharide had certain advantages in anti-inflammatory activity in the model of inhibiting the secretion of PGE2.The immunodulatory activity of two polysaccharides was produced mainly through promoting the secretion of TNF-α,but there was no significant difference between them in this model.
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