文章摘要
张倩,郭利伟,刘亚静,张咏鹤,徐光华,黄艳梅.河南新乡地区汉族群体MMPs基因启动子区5个SNPs与CHF发病风险关联性研究[J].济宁医学院学报,2018,41(1):48-52
河南新乡地区汉族群体MMPs基因启动子区5个SNPs与CHF发病风险关联性研究
Correlation of MMPs promoter region five single nucleotide polymorphisms with genetic susceptibility of CHF in Han population from Xinxiang city of Henan province
投稿时间:2017-11-09  
DOI:10.3969/j.issn.1000-9760.2018.01.013
中文关键词: 慢性充血性心力衰竭;基质金属蛋白酶;单核苷酸多态性;限制性扩增片段长度多态性
英文关键词: Matrix metallproteinases;Chronic heart failure;Single nucleotide polymorphism;Polymerase chain reaction restriction fragment length polymorphism
基金项目:河南省医学科技攻关计划项目(201403131);河南省教育科学“十二五”规划立项重点课题(2013-JKGHB-0035);新乡医学院大学生科研创新计划课题(2014-21)
作者单位E-mail
张倩 新乡医学院法医学院, 新乡 453003  
郭利伟 新乡医学院法医学院, 新乡 453003  
刘亚静 新乡医学院法医学院, 新乡 453003  
张咏鹤 新乡医学院法医学院, 新乡 453003  
徐光华 新乡医学院法医学院, 新乡 453003  
黄艳梅 新乡医学院法医学院, 新乡 453003 hym_cn@hotmail.com 
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中文摘要:
      目的 探讨河南新乡地区汉族群体基质金属蛋白酶基因启动子区rs2285053、rs11225395、rs225207、rs243865及rs11568818这5个单核苷酸多态性(SNPs)与心力衰竭(CHF)发病的相关性。方法 知情同意情况下,采集160例CHF患者(观察组)和186例健康体检者(对照组)外周静脉血提取基因组DNA,限制性扩增片段长度多态性方法对5个SNPs进行分型,运用SHEsis软件分析数据。结果 与对照组相比,MMPs基因rs2285053位点T等位基因的频率在观察组中显著降低(P<0.05); rs11225395位点的T等位基因和TT基因型频率在观察组中也显著降低(P<0.05),OR=1.966[95%CI(1.236~3.129)];而观察组中rs225207的G等位基因频率显著增加(P<0.05),OR=0.499[95%CI(0.320~3.799];rs2285053、rs11225395、rs225207组成的单体型CCG在观察组中的频率增高(P<0.05)。结论 rs11225395和rs2285053位点的等位基因T有可能降低CHF的患病风险;而rs225207位点的等位基因G及单体型CCG可能会增加CHF的患病风险。
英文摘要:
      Objective To assess the relationship between 5 SNPs including rs2285053,rs11225395,rs225207,rs243865 and rs11568818 at the promoter region of matrix metalloproteinases (MMPs)genes and the risk of chronic heart failure (CHF)in Han population from Xinxiang city of Henan province.Methods 5 SNPs sites of 160 CHF patients (observation group)and 186 healthyin dividuals (control group)were genotyped by PCR-RFLP methods.Then the data was analyzed by SHEsis software.Results The frequency of T allele of rs11225395 of patients in the observation group was significantly lower than that in the control group (P<0.05).The frequency of T allele and TT genotype of rs11225395 of patients in the observation group was lower than that in the control group (P<0.05)OR=1.96[95%CI (1.236~3.129)].The G allele of rs225207 of patients in the observation group was higher than that in the control group (P<0.05),OR=0.499[95%CI (0.320~3.799].The haplotype CCG consisting of rs2285053,rs11225395 and rs2285053 of patients in observation group was higher than those in the control group (P<0.05).Conclusion T allele of rs2285053 and rs11225395,G allele of rs225207 and the haplotype CCG may decrease the risk of CHF.
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