文章摘要
白波,程葆华,王春梅,潘衍有,陈京.Apelin-13对局灶性脑缺血-再灌注损伤大鼠行为和认知功能的影响[J].济宁医学院学报,2017,40(5):307-312
Apelin-13对局灶性脑缺血-再灌注损伤大鼠行为和认知功能的影响
The effects of Apelin-13 on the behavioral and cognitive function in the rat model of focal cerebral ischemia-reperfusion
投稿时间:2017-08-30  
DOI:10.3969/j.issn.1000-9760.2017.05.001
中文关键词: 缺血性脑卒中;Apelin-13;脑缺血-再灌注损伤;行为学;认知功能
英文关键词: Ischemic stroke;Apelin-13;Cerebral ischemia-reperfusion;Behavioral function;Cognitive function
基金项目:山东省科技发展计划(2012GGA08100);山东省自然科学基金(ZR2015CL021)。
作者单位E-mail
白波 济宁医学院神经生物学研究所, 济宁 272067 bbai@mail.jnmc.edu.cn 
程葆华 济宁医学院神经生物学研究所, 济宁 272067  
王春梅 济宁医学院神经生物学研究所, 济宁 272067  
潘衍有 济宁医学院神经生物学研究所, 济宁 272067  
陈京 济宁医学院神经生物学研究所, 济宁 272067  
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中文摘要:
      目的 观察侧脑室注射Apelin-13对脑缺血-再灌注大鼠模型(ischemia/reperfusion,I/R)行为学和认知功能的影响。方法 利用改进的Longa法制作大鼠大脑中动脉阻塞(middle cerebral artery occlusion,MCAO)模型。将SD大鼠随机分为假手术组、MCAO模型组、Apelin-13干预组,每组6只,疲劳转棒检测大鼠行为学变化,Morris水迷宫检测大鼠认知功能变化,免疫印迹检测脑源性神经营养因子(brain derived neurotrophic factor,BDNF)和Ras基因家族成员A(ras homolog gene family member A,RhoA)改变情况,ELISA检测乙酰胆碱酯酶(acetylcholinesterase,AChE)水平。结果 与假手术组相比较,MCAO模型大鼠转棒时间明显下降,认知功能受损,逃避潜伏期增加,穿台次数减少,BDNF表达下降,而RhoA表达和AChE活性升高,差异具有统计学意义(均P<0.05);与MCAO模型大鼠相比较,Apelin-13干预组运动功能改善,转棒时间明显延长,认知功能提升,逃避潜伏期降低,穿台次数增加,并抑制了BDNF表达的降低和RhoA表达的升高,减轻了AChE活性升高,差异具有统计学意义(均P<0.05)。结论 侧脑室注射Apelin-13可改善局灶性脑缺血-再灌注损伤大鼠行为和学习记忆功能,其机制可能是通过上调海马BDNF表达、抑制Rho A表达和AChE活性来实现。
英文摘要:
      Objective To investigate the effects of intracerebroventricular injection of Apelin-13 on the behavioral and cognitive function in the rat model of focal cerebral ischemia/reperfusion(I/R)induced by the middle cerebral artery occlusion(MCAO).Methods SD rats were divided into sham operation group,MCAO group and Apelin-13 intervention group.The behavioral function was measured by rota-rod.The cognitive function was measured by Morris water maze test.The expression of brain derived neurotrophic factor(BDNF)and ras homolog gene family member A(RhoA) in the hippocampus was detected by Western blotting.The activity of acetylcholinesterase(AChE) in the hippocampus was measured by ELISA.Results Compared with sham operation group,the time on the rota-rod was decreased significantly in MCAO group.And the cognitive function was impaired which was described by the increased escape latency and decreased the number to cross the original platform in the Morris water maze test.Subsequently,in the hippocampus the expression of BDNF was down-regulated,but the expression of RhoA was up-regulated significantly.The activity of acetylcholinesterase(AChE) in the hippocampus was increased markedly.Compared with MCAO group,Apelin-13 improved the behavioral function against the dysfunction of behavior induced by I/R damage through increasing the time on rota-rod.The impairment of cognitive function induced by I/R damage was reversed because of the intracerebroventricular injection of Apelin-13.The escape latency was shortened and the number to cross the original platform was increased compared with MCAO group.In addition,the up-regulation of BDNF and down-regulation of RhoA were inhibited due to Apelin-13 injection.The activity of AChE in the hippocampus was also decreased induced by Apelin-13 injection compared with MCAO group.Conclusion The intracerebroventricular injection of Apelin-13 improved the behavioral and cognitive function through regulating BDNF and Rho A expression in the hippocampus.
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