| 刘冰,卞晶晶,李晓琎,史才兴,鲍永华,管英俊.NeuroD1在ALS转基因鼠脊髓的表达[J].济宁医学院学报,2017,40(4):286-290 |
| NeuroD1在ALS转基因鼠脊髓的表达 |
| The expression of NeuroD1 in the spinal cord of ALS transgenic mice |
| 投稿时间：2017-02-24 |
| DOI：10.3969/j.issn.1000-9760.2017.04.013 |
| 中文关键词: 肌萎缩脊髓侧索硬化症;NeuroD1;神经退行性变;脊髓 |
| 英文关键词: Amyotrophic lateral sclerosis;NeuroD1;Neurodegeneration;Spinal cord |
| 基金项目:山东省科技发展计划（2012GSF11827）；国家自然科学基金资助项目（81271413） |
| 作者 | 单位 | E-mail | | 刘冰 | 济宁医学院精准医学研究院, 济宁 272067 | | | 卞晶晶 | 济宁医学院附属济宁市第一人民医院, 济宁 272011 | | | 李晓琎 | 济宁医学院基础医学院, 济宁 272067 | | | 史才兴 | 济宁医学院基础医学院, 济宁 272067 | | | 鲍永华 | 济宁医学院精准医学研究院, 济宁 272067 | | | 管英俊 | 潍坊医学院, 潍坊 261053 | guanyj@wfmc.edu.cn |
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| 中文摘要: |
| 目的 研究bHLH信号通路中激活型转录因子NeuroD1在肌萎缩脊髓侧索硬化症（amyotrophic lateral sclerosis，ALS）转基因模型鼠脊髓中的表达变化，进一步探讨bHLH信号通路在ALS转基因鼠发病中的作用机制，为临床治疗提供理论依据。方法 选取ALS转基因鼠和同窝出生的野生型鼠各18只，分别于95d、108d和122d取材，应用免疫荧光染色观测鼠脊髓中NeuroD1阳性细胞数，RT-PCR和Western blot分别检测鼠脊髓NeuroD1 mRNA及蛋白水平。结果 免疫荧光实验结果显示，NeuroD1阳性细胞主要分布在脊髓灰质前角，与同窝野生型鼠比较，ALS转基因鼠NeuroD1阳性细胞明显减少（t=6.82，P< 0.05；t=17.22，P< 0.05；t=5.86，P< 0.05）。与同窝野生型鼠比较，ALS转基因鼠NeuroD1 mRNA（t=3.82，P< 0.05；t=5.85，P< 0.05；t=4.96，P< 0.05）和蛋白表达在95d、108d和122d均降低（t=8.49，P< 0.05；t=8.74，P< 0.05；t=5.94，P< 0.05）。结论 在ALS转基因鼠发病脊髓中NeuroD1阳性细胞明显减少，NeuroD1 mRNA和蛋白表达降低，表明NeuroD1与ALS的发生密切相关。 |
| 英文摘要: |
| Objective This study was designed to determine the expression of bHLH signaling transcription factor NeuroD1 in spinal cord of amyotrophic lateral sclerosis (ALS) transgenic mice,and further to explore the mechanism of NeuroD1 in the pathogenesis of ALS transgenic mice.Methods Eighteen mice from each group of the ALS and wild-type adult mice were sacrificed at the age of 95d,108d,and 122d,respectively.NeuroD1 positive cells were extracted and analyzed with immunofluorescence technique.And NeuroD1 mRNA and total protein were extracted and analyzed with Western blotting and quantitative RT-PCR.Results Immunofluorescence results showed that NeuroD1-positive cells were reduced in the spinal cord of ALS transgenic mice at different ages compared with wild-type mice (P<=6.82,P<0.05;P<=17.22,P<0.05;t=5.86,P<0.05).Compared with wild-type mice,the expression of NeuroD1 mRNA (P<=3.82,P<0.05;P<=5.85,P<0.05;P<=4.96,P<0.05) and protein levels were decreased at 95d,108d and 122d in the ALS transgenic mice(P<=8.49,P<0.05;P<=8.74,P<0.05;P<=5.94,P<0.05).Conclusion NeuroD1-positive cells and the expression of NeuroD1 mRNA and protein were all decreased in the spinal cord of ALS transgenic mice,suggesting that NeuroD1 was closely related to the pathogenesis of amyotrophic lateral sclerosis. |
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