| 崔亚男,郭富双,管华,孙珊珊,王慧云.牛血清白蛋白温敏凝胶给药系统的制备及释放度考察[J].济宁医学院学报,2017,40(4):275-277,281 |
| 牛血清白蛋白温敏凝胶给药系统的制备及释放度考察 |
| Study on the preparation and release properties of bovine serum albumin loaded temperature-sensitive gel |
| 投稿时间：2017-07-11 |
| DOI：10.3969/j.issn.1000-9760.2017.04.010 |
| 中文关键词: 温敏凝胶;牛血清白蛋白;普朗尼克F127;缓-控释制剂 |
| 英文关键词: Temperature-sensitive gel;Bovine serum albumin;Pluoronic F127;Modified-release preparation |
| 基金项目:济宁医学院科研计划重点项目（JY2013KJ 002）；济宁医学院青年基金项目（JYQ2011 KM028） |
| 作者 | 单位 | E-mail | | 崔亚男 | 济宁医学院药学院, 日照 276826 | | | 郭富双 | 济宁医学院药学院, 日照 276826 | | | 管华 | 济宁医学院药学院, 日照 276826 | | | 孙珊珊 | 济宁医学院药学院, 日照 276826 | | | 王慧云 | 济宁医学院药学院, 日照 276826 | wang_huiyun@126.com |
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| 摘要点击次数: 2971 |
| 全文下载次数: 1985 |
| 中文摘要: |
| 目的 制备牛血清白蛋白（BSA）温敏凝胶给药系统，并考察给药系统的释放情况。方法 以温敏凝胶材料普朗尼克F127为载体，以相变温度为指标，通过倒置试管法筛选普朗尼克F127的浓度。通过BCA蛋白定量试剂盒法测定BSA的含量。以PBS缓冲液为释放介质，考察凝胶给药系统中BSA的释放。结果 浓度为20%（w/v）的普朗尼克F127凝胶的相变温度为23℃，载入BSA后相变温度不变。BSA在普朗尼克F127凝胶系统中的释放12h可释放80%以上，具有缓释效果，且释放接近零级动力学过程。结论 BSA-普朗尼克F127温敏凝胶系统制备方法简单，具有明显的缓释效果。 |
| 英文摘要: |
| Objective To study on the preparation of bovine serum albumin (BSA) loaded temperature-sensitive gel and the release property of BSA.Methods Pluoronic F127 was selected as carriers.The concentration of pluoronic F127 was optimized through the inverted test tube method using the phase-transition temperature as the index.The concentration of BSA was determined using BCA kits.The release property of BSA was investigated using PBS as the dissolution media.Results The phase-transition temperature of pluoronic F127 with the concentration of 20% (w/v) was 23℃ and retained identically when BSA was loaded.The results of release showed that after 12h,more than 80% of BSA was released and the release property was nearly zero-order kinetics.Conclusion The preparation technique of BSA loaded pluoronic F127 temperature-sensitive gel is feasible.The release of BSA could be well controlled. |
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