| 李晓琎,史才兴,柳新平,徐旭东.芒果苷对小鼠肝缺血-再灌注损伤的保护作用[J].济宁医学院学报,2017,40(3):205-208,213 |
| 芒果苷对小鼠肝缺血-再灌注损伤的保护作用 |
| Protective effects of mangiferin on hepatic ischemia-reperfusion injury in mice |
| 投稿时间：2017-01-09 |
| DOI：10.3969/j.issn.1000-9760.2017.03.013 |
| 中文关键词: 芒果苷;缺血-再灌注;肝脏 |
| 英文关键词: Mangiferin;Ischemia-reperfusion injury;Liver |
| 基金项目:济宁医学院科研计划项目（JY2015KJ008）；济宁医学院青年教师科研扶持基金（JY2016KJ021Y） |
| 作者 | 单位 | E-mail | | 李晓琎 | 济宁医学院基础医学院, 济宁 272067 | | | 史才兴 | 济宁医学院基础医学院, 济宁 272067 | shicaixing123@126.com | | 柳新平 | 济宁医学院基础医学院, 济宁 272067 | | | 徐旭东 | 济宁医学院基础医学院, 济宁 272067 | |
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| 中文摘要: |
| 目的 探讨芒果苷对小鼠肝缺血-再灌注损伤的保护作用及其作用机制。方法 成年昆明小鼠24只，体重18~20g，随机分成缺血-再灌注损伤组（I/R组）、芒果苷组和假手术组（sham组）。I/R组采用夹闭肝蒂30min、再灌注6h制作肝缺血-再灌注损伤模型。芒果苷组于缺血前30min时腹腔注射芒果苷20mg/kg；sham组和I/R组腹腔注射等容量生理盐水。再灌注6h时，取材，HE染色，光学显微镜下观察肝细胞形态学变化；比色法检测血清丙氨酸氨基转移酶（ALT）的水平及肝组织中超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽过氧化物酶（GSH-Px）的活性；Western blot检测肝组织Caspase-3、Bcl-2、Bax蛋白表达。结果 经芒果苷处理后，芒果苷组与I/R组相比肝细胞形态学异常明显减轻；血清ALT（133.73±11.53）含量和肝组织MDA（7.59±0.97）含量较I/R组明显降低，差异有统计学意义（t=-7.22，P< 0.05；t=-3.07，P< 0.05）；肝组织SOD（119.40±13.95）和GSH-Px（97.59±14.07）含量较I/R组升高，差异有统计学意义（t=3.34，P< 0.05；t=3.32，P< 0.05）。经芒果苷处理后，芒果苷组与I/R组相比肝组织Caspase-3及Bax蛋白表达降低，差异有统计学意义（t=-3.18，P< 0.05；t=-8.86，P< 0.05）；Bcl-2蛋白表达升高，差异有统计学意义（t=4.47，P< 0.05）。结论 芒果苷对小鼠肝缺血-再灌注损伤具有保护作用，其保护机制可能与其抗氧化作用和抑制细胞凋亡有关。 |
| 英文摘要: |
| Objective To investigate the protective effects of mangiferin on hepatic ischemia-reperfusion (I/R) injury in mice.Methods Twenty-four healthy Kunming mice,weighing 18~20g,were randomly divided into three groups:I/R,mangiferin and sham.The hepatic I/R injury model of I/R group was clamped the hepatic artery,portal vein,and common bile duct with a microvascular bulldog clamp for 30 min,and then removed the clamp and reperfused for 6h.In mangiferin group,20mg/kg mangiferin was injected intraperitoneally before 30min of ischemia.The equal volume of normal saline was given instead of mangiferin in sham and I/R groups.At 6h of reperfusion,blood samples were collected from the heart and then the hepatic tissue was taken out.Hepatic morphology of hematoxylin-eosin (HE) dying were observered by microscope.The activation of aspartate aminotransferase (AST),superoxide dismutase (SOD),malondialdehyde (MDA),and glutathione peroxidase (GSH-Px) was measured by the OD values.The expression of Caspase-3,Bcl-2 and Bax protein in the hepatic tissues were detected by Western blot.Results Mangiferin rescued hepatocyte damage by morphological observation.Mangiferin contents of ALT (135.59±19.07) and MDA (7.59±0.97) were lower than those in I/R group and the difference was statistically significant (t=-7.22,P< 0.01;t=-3.07,P< 0.01).The activities of SOD (119.40±13.95) and GSH-Px (97.59±14.07) in the hepatic tissues were higher than those in I/R group,and the difference was statistically significant (t=3.34,P< 0.01;t=3.32,P< 0.01).Mangiferin contents of Caspase-3 and Bax protein expression levels were lower than those in I/R group,and the difference was statistically significant (t=-3.18,P< 0.01;t=-8.86,P< 0.01).And the expression of Bcl-2 protein were increased than that in I/R group,and the difference was statistically significant (t=4.47,P< 0.01).Conclusion Mangiferin can significantly protect the hepatic tissue against the I/R induced injury.The mechanism may be related to the effect of anti-oxidant and inhibiting cell apoptosis. |
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