| 戴伟娟,朱凡河,张国安,徐旭东,王旭.前列腺素E1对大鼠全脑缺血-再灌注损伤细胞凋亡及Caspase-3表达的影响[J].济宁医学院学报,2017,40(2):98-102 |
| 前列腺素E1对大鼠全脑缺血-再灌注损伤细胞凋亡及Caspase-3表达的影响 |
| Effects of PGE1 on the apoptosis and expression of caspase-3 in rats with cerebral ischemia-reperfusion injury |
| 投稿时间：2017-03-09 |
| DOI：10.3969/j.issn.1000-9760.2017.02.005 |
| 中文关键词: 前列腺素E1;脑缺血-再灌注;细胞凋亡;半胱氨酸天冬氨酸蛋白酶3 |
| 英文关键词: Prostaglandin E1;Rat;Cerebral ischemia-reperfusion;apoptosis;Caspase-3 |
| 基金项目:山东省自然科学基金项目(2012ZRB14146) |
| 作者 | 单位 | | 戴伟娟 | 济宁医学院基础医学院, 济宁 272067 | | 朱凡河 | 济宁医学院基础医学院, 济宁 272067 | | 张国安 | 济宁医学院基础医学院, 济宁 272067 | | 徐旭东 | 济宁医学院基础医学院, 济宁 272067 | | 王旭 | 济宁医学院基础医学院, 济宁 272067 |
|
| 摘要点击次数: 9494 |
| 全文下载次数: 4705 |
| 中文摘要: |
| 目的 探讨前列腺素E1（PGE1）对大鼠全脑缺血-再灌注损伤神经元凋亡和半胱氨酸天冬氨酸蛋白酶3 （Caspase-3）表达的影响。方法 将健康雄性Wistar大鼠24只，随机分为3组：假手术组、缺血-再灌模型组、PGE1组（12μg/kg），药物于缺血前5mins静脉滴注，持续2h；利用双侧颈总动脉结扎法建立大鼠全脑缺血-再灌注模型，形态学方法观察细胞凋亡的变化，免疫组化染色方法检测脑组织Caspase-3，表达的变化。结果 缺血20mins，再灌注6h后，缺血-再灌模型大鼠脑组织细胞凋亡数和Caspase-3的表达，较假手术组明显增多（P <0.05），与缺血-再灌注模型组相比，PGE1组能减少细胞凋亡的数量，抑制Caspase-3的表达（P <0.05）。结论 PGE1脑保护作用机制与其抑制神经细胞凋亡和Caspase-3表达有关。 |
| 英文摘要: |
| Objective To investigate the effects of Prostaglandin E1(PGE1) on the neuronal apoptosis and expression of caspase-3 in rats with whole brain ischemia-reperfusion injury.Methods 24 healthy male Wistar rats were randomly divided into three groups which were sham operated group (n=8),cer ebral ischemia-reperfusion model group(n=8).PGE1-pretreated groups (12μg/kg,n=8).Rats model of whole cerebral ischemia-reperfusion injury were made through bilateral common carotid artery ligation for 20min followed with 6 hours of reperfusion.The change of cellular apoptosis were observed with morphological method,and the expression of caspase-3 were detected using immunohistochemical method.Results After the ischemia for 20 min and reperfusion for 6 hin rats,the number of apoptotic cells and the expressions of caspase-3 in brain tissue were increased compared with those in the sham operative group (P <0.01).In PGE1 group,the number of apoptosis cells and the expressions of caspase-3 were lower than those in the isehemia reperfusion model group (P <0.01).Conclusion The protective mechanism of PGE1 on cerebral ischemia-reperfusion injury is related to reducing neuronal apoptosis by inhibiting the expression of caspase-3. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
