| 李丹,张金国.大鼠骨髓间充质干细胞体外培养体系的建立[J].济宁医学院学报,2015,(5):321-323 |
| 大鼠骨髓间充质干细胞体外培养体系的建立 |
| Establishment of an in vitro culture system of rat bone marrow mesenchymal stem cells |
| 投稿时间：2015-09-09 |
| DOI：10.3969/j.issn.1000-9760.2015.05.005 |
| 中文关键词: 干细胞|骨髓间充质干细胞|培养|鉴定 |
| 英文关键词: Stem cells|Bone marrow mesenchymal stem cells|Culture|Identification |
| 基金项目: |
| 作者 | 单位 | E-mail | | 李丹 | 济宁医学院2013级研究生, 山东济宁 272067
济宁医学院附属医院, 济宁 272029 | | | 张金国 | 济宁医学院2013级研究生, 山东济宁 272067
济宁医学院附属医院, 济宁 272029 | cck112000@aliyun.com |
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| 中文摘要: |
| 目的 本文旨在介绍大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)体外培养体系建立的一套完整方法,及操作中的相关注意事项。方法 体重100g左右健康清洁SD大鼠,取双侧股骨及胫骨,冲出骨髓腔细胞后培养,72h后全量换液,待细胞融合至80%左右时传代培养,取生长良好的P3代细胞通过流式细胞仪,进行CD90、CD45表型鉴定。结果 体外培养P3代细胞表面标记物CD90、CD45鉴定完全符合标准,BMSCs纯化并大量扩增。结论 通过全骨髓贴壁培养法可以建立一套完整的体外培养体系。 |
| 英文摘要: |
| Objective To introduce a complete set of methods for the establishment of rat bone marrow mesenchymal stem cells in vitro culture system and the relevant matters needing attention in the operation.Methods We selected healthy clean SD rats about 100g,cut out the bilateral femur and tibia,and developed the cells out of the bone marrow cavity.The cell culture fluid was all changed in 72 hours.When the cell fusion grew to 80%,we used trypsin for digestion and passaging the culture.The expression of CD90 and CD45 in P3 cells was identified by flow cytometry.Results The cell surface antigens CD90 and CD45 of in vitro cultured P3 cells were all in line with the standard.BMSCs were purified and amplified in vitro.Conclusion A complete set of in vitro culture system was established by the method of whole bone marrow adherent culture. |
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