| 孙恺,谢楠,陶庆霞,王翀.抗HMGB1单抗对小鼠脑缺血-再灌注损伤后IL-1β IL-6表达的影响[J].济宁医学院学报,2015,(5):313-317 |
| 抗HMGB1单抗对小鼠脑缺血-再灌注损伤后IL-1β IL-6表达的影响 |
| Effect of anti-HMGB1 monoclonal antibody on the expression of IL-1β and IL-6 after brain ischemia reperfusion |
| 投稿时间：2015-06-29 |
| DOI：10.3969/j.issn.1000-9760.2015.05.003 |
| 中文关键词: 脑缺血-再灌注损伤|炎症因子|高迁移率族蛋白B1|单克隆抗体 |
| 英文关键词: Cerebral ischemic reperfusion injury|Inflammatory factors|High mobility group protein B1 (HMGB1)|Monoclonal antibody |
| 基金项目: |
| 作者 | 单位 | | 孙恺 | 潍坊医学院2013级研究生, 山东潍坊 261053 | | 谢楠 | 徐州市中心医院, 江苏徐州 221000 | | 陶庆霞 | 潍坊医学院2013级研究生, 山东潍坊 261053 | | 王翀 | 济宁医学院附属济宁市第一人民医院, 山东济宁 272011 |
|
| 摘要点击次数: 4905 |
| 全文下载次数: 985 |
| 中文摘要: |
| 目的 探讨抗高迁移率族蛋白B1(high mobility group protein box 1,HMGB1)单抗(mAb)对小鼠脑缺血-再灌注损伤后炎症因子IL-1β、IL-6 mRNA表达的影响。方法 采用大脑中动脉阻断术 (MCAO) 建立小鼠脑缺血-再灌注损伤模型,造模成功后随机分为假手术组(sham组),脑缺血-再灌注组(I/R组),抗HMGB1单克隆抗体组(mAb组)和单抗对照组(control IgG组),mAb组小鼠经尾静脉注射30μg/g抗HMGB1单抗,control IgG组尾静脉注射同等剂量小鼠IgG;ELISA测定血清HMGB1浓度;干湿重法测定脑水肿程度;HE染色观察病理形态学变化;实时荧光定量PCR测定小鼠脑组织IL-1β,IL-6 mRNA的表达量变化。结果 1)ELISA测得I/R小鼠血清HMGB1的浓度为(47.75±3.667)ng/ml,明显高于sham组小鼠的HMGB1浓度(5.353±1.489)ng/ml(t=10.71,P<0.001);2)I/R组、mAb组脑组织含水量分别为 (78.34±2.836)%、(65.09±1.934)%,I/R组显著高于mAb组(t=3.86,P<0.05);3)mAb组脑组织缺血损伤病理学改变明显轻于I/R组;4)I/R组IL-1βmRNA表达量(0.783±0.028)显著高于mAb组(0.417±0.047) (t=22.08,P<0.01),mAb组IL-6MRNA表达量(0.655±0.052)与I/R组(0.650±0.052)相比,无明显差异 (t=0.089,P>0.05)。结论 HMGB1介导脑缺血-再灌注损伤机制中炎症反应过程,小鼠静脉注射抗HMGB1单抗可有效保护受损脑组织。 |
| 英文摘要: |
| Objective To investigate the effect of anti-HMGB1 monoclonal antibody on the expression of IL-1β and IL-6 after brain ischemia reperfusion.Methods The models were established by an intraluminal suture method to induce brain ischemia reperfusion injury.Animals were randomly divided into sham group, ischemia reperfusion (I/R) group, anti-HMGB1 monoclonal antibody (mAb) group (30μg/g), and IgG control group.After that, serum HMGB1 levels were measured by ELISA analysis, Brain edema was investigated by dry-wet method.H&E staining was used to observe pathologic changes, and the expressions of IL-1β and IL-6 were tested by real-time PCR.Results 1) The concentrations of serum HMGB1 in I/R group[(47.75±3.667)ng/ml]were significantly higher than that of sham group[(5.353±1.489)ng/ml] (t=10.71,P<0.0001).2) The brain water content in I/R group[(78.34±2.836)%] was higher than mAb group[(65.09±1.934)%] (t=3.86,P<0.05); 3) I/R group presented worse damage to the brain compared with mAb group showed by H&E staining.4) The expressions of IL-1β in I/R group (0.783±0.028) were significantly increased by contrast with mAb group (0.417±0.047) (t=22.08, P<0.01).There was no significant difference in the expressions of IL-6 between the 2 groups (t=0.089,P>0.05).Conclusion HMGB1 mediates the process of inflammatory reaction in brain ischemia reperfusion.Anti-HMGB1 monoclonal antibody contributes to the protection against ischemia reperfusion injury in brain. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
