| 武菲,白波,张秋玲.体外培养皮层神经元氧糖剥夺致细胞凋亡模型的建立[J].济宁医学院学报,2015,(1):19-22 |
| 体外培养皮层神经元氧糖剥夺致细胞凋亡模型的建立 |
| Induction of apoptosis by oxygen-glucose deprivation of primary cortical neurons in vitro |
| 投稿时间：2015-01-15 |
| DOI：10.3969/j.issn.1000-9760.2015.01.004 |
| 中文关键词: 氧糖剥夺;凋亡;模型 |
| 英文关键词: Oxygen-Glucose Deprivation;Apoptosis;Model |
| 基金项目:2014年济宁医学院青年基金项目(编号:JYQ14KJ19) |
| 作者 | 单位 | | 武菲 | 济宁医学院基础学院, 山东济宁 272067 | | 白波 | 济宁医学院基础学院, 山东济宁 272067 | | 张秋玲 | 泰山医学院, 山东泰安 271000 |
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| 中文摘要: |
| 目的 采用简便、有效的方法建立体外培养神经元氧糖剥夺致凋亡的模型。方法 新生大鼠皮层神经元培养7d后,全量换液为不含葡萄糖的Earle's液,并以灭菌液体石蜡封闭。MAP-2荧光染色观察神经元损伤情况,采用LDH和Tunel法检测大鼠皮层神经元大致死亡和凋亡情况。结果 随着氧糖剥夺时间的延长,神经元损伤加重并发生凋亡,与对照组比较有显著差异。结论 无糖Earle's液培养并配合灭菌液体石蜡封闭,可以模拟神经元缺血缺氧,导致神经元凋亡。 |
| 英文摘要: |
| Objective To establish an effective and convenient neuronal apoptosis model in vitro induced by oxygen-glucose deprivation.Methods The cortical neurons of newborn rats'was cultured for 7 days.The whole cell culture medium was removed and then Earle's solution with glucose or without glucose was added.The glucose-free Earle's solution was sealed with sterile paraffin liquid.The damage of neurons were observed by MAP-2 fluorescent staining,while the neuronal death and apoptosis were investigated by LDH release and TUNEL staining.Results LDH release increased in a time-dependent manner,and the apoptosis could be induced by OGD as showed by TUNEL staining.There was significant difference between OGD and control group.Conclusion It is feasible to imitate the hypoxia ischemia of neurons in vitro which induces the apoptosis via culturing with glucose-free Earle's solution and sealing with sterile paraffin liquid. |
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