| 辛青,程葆华,王琳,陈京,白波.炎性因子在脑缺血-再灌注不同时期的基因表达[J].济宁医学院学报,2014,(4):240-243 |
| 炎性因子在脑缺血-再灌注不同时期的基因表达 |
| The gene expression of pro-inflammatory cytokines at different durations of cerebral ischemia reperfusion |
| 投稿时间：2014-06-11 |
| DOI：10.3969/j.issn.1000-9760.2014.04.003 |
| 中文关键词: 脑缺血;炎症反应;神经损伤 |
| 英文关键词: Cerebral ischemia;Inflammation;Neural damage |
| 基金项目:山东省自然科学基金项目(编号:2012GSF11806);国家自然科学基金项目(编号:81070961) |
| 作者 | 单位 | E-mail | | 辛青 | 新乡医学院基础学院, 河南新乡 453003
济宁医学院神经生物学研究所, 山东济宁 272067 | | | 程葆华 | 济宁医学院神经生物学研究所, 山东济宁 272067 | | | 王琳 | 济宁医学院神经生物学研究所, 山东济宁 272067 | | | 陈京 | 济宁医学院神经生物学研究所, 山东济宁 272067 | | | 白波 | 济宁医学院神经生物学研究所, 山东济宁 272067 | bbai@mail.jnmc.edu.cn;chenjnmc@163.com |
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| 中文摘要: |
| 目的 探讨白介素-1β(IL-1β)、肿瘤坏死因子α(TNF-α)和细胞间黏附分子1(ICAM-1)在脑缺血-再灌注不同时期的基因表达。方法 将Wistar大鼠随机分为假手术组和脑缺血30 min再灌注6 h(IR6h)、12 h(IR12h)、24 h(IR24h)、48 h(IR48h)组。动物模型采用线栓法制备大鼠中动脉栓塞法,用RT-PCR法检测脑缺血－再灌注不同时间点IL-1β、TNF-α及ICAM-1的表达。结果 IL-1β、TNF-α及ICAM-1在假手术组脑组织内低水平表达。IL-1β mRNA的表达在脑缺血-再灌注6 h后即明显增高,12 h后表达开始减弱,24~48 h呈低水平增高。TNF-α在脑缺血-再灌注6 h表达开始上升,24 h达到高峰,48 h后开始下降。ICAM-1脑缺血-再灌注6 h表达开始升高,24 h达到高峰,48 h后仍维持较高水平。结论 炎症因子参与了脑缺血-再灌注性损伤,且具有明显的时间规律。 |
| 英文摘要: |
| Objective To explore the gene expression of pro-inflammatory cytokines after different durations of cerebral ischemia reperfusion.Methods Rats were randomly divided into five groups:sham group,30min of cerebral ischemia after 6h,12h,24h and 48h groups.Transient focal ischemia/reperfusion model in male Wistar rats were induced by middle cerebral artery occlusion(MCAO) according to the intraluminal filament technique as described.The expression of pro-inflammatory cytokines including interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α) and intercellular adhesion molecule-1(ICAM-1) were measured using RT-PCR.Results The gene expression of IL-1β,TNF-α,ICAM-1 was low in sham group.The mRNA expression of IL-1β was significantly increased and reached the peak after 6h,but began a decrease after 12h and kept the low level at 24h and 48h.The mRNA expression of TNF-α gradually increased after 6 h,and reached the highest levels at 24h,then began to decrease at 48h.The mRNA expression of ICAM-1 gradually increased following cerebral ischemia,and reached the peak at 24h and 48h.Conclusion Our results demonstrated that cerebral ischemia reperfusion could induce the up-regulation of IL-1β,TNF-α and ICAM-1 in a time-dependent way. |
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