| 张新欣,李晓丽,庄珊,刘深,李秀华,李衍滨.青蒿琥酯对实验性自身免疫性重症肌无力大鼠的免疫调节作用[J].济宁医学院学报,2014,(3):165-168 |
| 青蒿琥酯对实验性自身免疫性重症肌无力大鼠的免疫调节作用 |
| The effect of artesunate on experimental autoimmune myasthenia gravis in rats |
| 投稿时间：2014-03-29 |
| DOI：10.3969/j.issn.1000-9760.2014.03.004 |
| 中文关键词: 青蒿琥酯;实验性自身免疫性重症肌无力;TNF-α;IgG2a;IgG2b |
| 英文关键词: Artesunate;Experimental autoimmune myasthenia gravis;TNF-α;IgG2a;IgG2b |
| 基金项目:山东省自然基金(编号:2010ZRA01101);山东省优秀中青年科学家科研奖励基金(编号:2008BS03012) |
| 作者 | 单位 | E-mail | | 张新欣 | 潍坊医学院临床学院, 山东潍坊 261053 | | | 李晓丽 | 山东大学附属千佛山医院, 山东济南 250014 | | | 庄珊 | 山东中医药大学, 济南 250014 | | | 刘深 | 山东中医药大学, 济南 250014 | | | 李秀华 | 山东大学附属千佛山医院, 山东济南 250014 | | | 李衍滨 | 山东大学附属千佛山医院, 山东济南 250014 | lch1226@sohu.com |
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| 中文摘要: |
| 目的 探究青蒿琥酯对实验性自身免疫性重症肌无力(experimental autoimmune myasthenia gravis,EAMG)大鼠TNF-α、IgG2a、IgG2b的影响。方法 用人工合成的大鼠来源的乙酰胆碱受体(acetylcholine receptor,AChR)α亚基97-116肽段免疫Lewis大鼠制造重症肌无力(myasthenia gravis,MG)模型,随机分为青蒿琥酯大剂量组(100 mg/kg)、小剂量组(10 mg/kg)和模型对照组,流式细胞仪检测不同组大鼠淋巴结单个核细胞(mononuclear cell,MNC)中TNF-α+细胞的百分比;酶联免疫吸附法(ELISA)检测EAMG大鼠血清中抗R97-116 IgG2a和IgG2b抗体的水平。结果 与对照组相比,青蒿琥酯小剂量组明显下调了TNF-α+的百分比(P=0.046),而大剂量组中TNF-α下调不显著,两治疗组血清中抗R97-116 IgG2a和IgG2b抗体的水平下降,且小剂量组血清中抗R97-116 IgG2a和IgG2b抗体的水平下降更显著。结论 青蒿琥酯主要是通过下调TNF-α+细胞的数量和降低血清中抗R97-116 IgG2a和IgG2b抗体水平来改善EAMG大鼠的病情。 |
| 英文摘要: |
| Objective To investigate the effect of artesunate on TNF-α,IgG2a,IgG2b of experimental autoimmune myasthenia gravis(EAMG) in rats.Methods EAMG can be induced by subcutaneous injection into both hind footpads with a synthetic peptide corresponding to region 97-116 of the rat AChR α subunit(R97-116 peptide).Artesunate was administered by gavage to EAMG rats.The percentage of TNF-α+ cells among lymph nodes(mononuclear cell,MNC) was analyzed by flow cytometry.Anti-R97-116 peptide IgG2a and IgG2b antibodies in serum were detected by ELISA.Results On the day of the experiment termination,the rats in 10 mg/kg artesunate-treated group exhibited less percentage of TNF-α+ cells and lower level of anti-R97-116 IgG antibodies(P<0.05) compared with rats in control group.The therapeutic effect of artesunate on EAMG rats was associated with down-regulated TNF-α+ cells(P<0.05) and decreased anti-R97-116 IgG antibody(P<0.01).Conclusion Artesunate could ameliorate EAMG by down-regulating the number of TNF-α+ cells and decreasing anti-R97-116 IgG antibody. |
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