| 吴彬,孟纯阳,王海滨,贾存岭,赵益峰,贾代良.颈椎黄韧带叠压症兔黄韧带TGF-β1的表达[J].济宁医学院学报,2013,(6):393-395,399 |
| 颈椎黄韧带叠压症兔黄韧带TGF-β1的表达 |
| Experimental research of TGF-β1 expressed in folding compression of cervical ligamentum flavum animal models |
| 投稿时间：2013-10-22 |
| DOI：10.3969/j.issn.1000-9760.2013.06.005 |
| 中文关键词: TGF-β1;颈椎黄韧带叠压;动物模型 |
| 英文关键词: TGF-β1;folding compression of cervical ligamentum flavum;animal models |
| 基金项目:济宁医学院青年基金项目(编号:20090511) |
| 作者 | 单位 | | 吴彬 | 济宁医学院附属医院, 山东济宁 272029 | | 孟纯阳 | 济宁医学院附属医院, 山东济宁 272029 | | 王海滨 | 济宁医学院附属医院, 山东济宁 272029 | | 贾存岭 | 济宁医学院附属医院, 山东济宁 272029 | | 赵益峰 | 济宁医学院附属医院, 山东济宁 272029 | | 贾代良 | 济宁医学院附属医院, 山东济宁 272029 |
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| 中文摘要: |
| 目的 研究颈椎黄韧带叠压兔黄韧带中转化生长因子β1(transforming growth factor-β1,TGF-β1)的表达变化。方法 48只新西兰大白兔,随机分为模型组和对照组,每组24只动物。模型组通过颈椎前路穿刺破坏纤维环及抽吸颈5/6髓核组织建立兔颈椎黄韧带叠压动物模型,术后4、8、12周3个时间点处死动物取材,切取颈5/6后方黄韧带组织,制作石蜡切片并做免疫组织化学检查及图像分析。结果 模型组黄韧带纤维排列紊乱,玻璃样变性,TGF-β1表达升高,与对照组相比差异有显著统计学意义(P<0.05),其中术后4周组变化最为明显,术后8、12周TGF-β1的表达较术后4周降低,差异无统计学意义。结论 TGF-β1在颈椎黄韧带叠压兔黄韧带中的表达增加,加速了黄韧带的退变。 |
| 英文摘要: |
| Objective To study TGF-β1 expressed in folding compression of cervical ligamentum flavum animal models.Methods Forty-eight mature New Zealand white rabbits (3000±250g) were divided into two groups randomly,with 24 rats in control group and 24 rats in model group.The folding compression of cervical ligamentum flavum animal models were made by destroyed annulus fibrosus and absorbed nucleus pulposus.Eight rabbits in each group were killed four weeks,eight weeks and twelve weeks after operation.The ligamentum flavum of C5/6 were collected,embedded in paraffin and cut into slices.The slices were treated by immunohistochemistry and the image analysis.Results Fibers derangement and hyaline degeneration in ligamentum flavum was found in model group.The expression of TGF-β1 in model group was higher than control group,and the difference was significant(P<0.05).After operation for 8 and 12 weeks in the model group the expression of TGF-β1was decreased than those of 4 weeks.But there was no significant difference in statistics.Conclusion Expression of TGF-β1 increased in folding compression of cervical ligamentum flavum animal models and accelerate the degeneration of the ligamentum flavum. |
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