| 杨海霞,张孝侠,程晓平,翟景花,方召阳.金银花和野菊花乙醇提取物抑菌作用比较[J].济宁医学院学报,2013,(2):97-99,105 |
| 金银花和野菊花乙醇提取物抑菌作用比较 |
| Comparative study on bacteriostatic efficacy of honeysuckle and wild chrysanthemum |
| 投稿时间：2013-02-11 |
| DOI：10.3969/j.issn.1000-9760.2013.02.006 |
| 中文关键词: 金银花乙醇提取物;野菊花乙醇提取物;抑菌作用 |
| 英文关键词: Honeysuckle extract;Wild chrysanthemum extract;Bacteriostatic efficacy |
| 基金项目: |
| 作者 | 单位 | | 杨海霞 | 济宁医学院法医学与医学检验学院, 济宁山东 272067 | | 张孝侠 | 济宁医学院法医学与医学检验学院, 济宁山东 272067 | | 程晓平 | 济宁医学院法医学与医学检验学院, 济宁山东 272067 | | 翟景花 | 济宁医学院法医学与医学检验学院, 济宁山东 272067 | | 方召阳 | 济宁医学院法医学与医学检验学院, 济宁山东 272067 |
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| 中文摘要: |
| 目的 研究金银花和野菊花乙醇提取物抑菌作用的差异。方法 采用抑菌环试验对它们的抑菌作用进行观察比较。结果 生药含量为492mg/ml金银花乙醇提取物对金黄色葡萄球菌、大肠杆菌的抑菌环直径均大于8mm,而对枯草杆菌的抑菌环直径小于8mm;生药含量为1035mg/ml野菊花乙醇提取物对金黄色葡萄球菌、枯草杆菌的抑菌环直径均大于8mm,而对大肠杆菌的抑菌环直径小于8mm;金银花乙醇提取物对金黄色葡萄球菌和枯草杆菌抑菌作用较强,两种菌的最低抑菌浓度皆为1:8,对大肠杆菌的最低抑菌浓度为1:4;野菊花乙醇提取物对大肠杆菌抑菌作用最强,最低抑菌浓度为1:64;对金黄色葡萄球菌的最低抑菌浓度为对枯草杆菌的最低抑菌浓度为1:16;对枯草杆菌的抑菌作用最弱,最低抑菌浓度为1:4。结论 金银花乙醇提取物对金黄色葡萄球菌、枯草杆菌有抑菌作用,而对大肠杆菌无抑菌作用;野菊花乙醇提取物对金黄色葡萄球菌、大肠杆菌有抑菌作用,而对枯草杆菌无抑菌作用。 |
| 英文摘要: |
| Objective The bacteriostatic efficacy of alcohol extract of honeysuckle and wild chrysanthemum were investigated in this work. Methods Their reaction was observed and compared by experiments with Paper Dish methods. Results The diameters of bacterial inhibition zones of two bacteria such as Staphylococcus aureus and Subtilis Bacillus to the ethanol extract containing honeysuckle crude drug 492mg/ml were larger than 8mm, but the diameters of bacterial inhibition zones of Escherichia coliand Bacillus to the ethanol extract containing honeysuckle crude drug 492mg/ml were smaller than 8mm. The diameters of bacterial inhibition zones of two bacteria such as Staphylococcus aureus and Escherichia coliand Bacillus to the ethanol extract containing wild chrysanthemum drug 1035mg/ml were larger than 8mm, but the diameters of bacterial inhibition zones of Subtilis Bacillus to the ethanol extract containing wild chrysanthemum drug 1035mg/ml were smaller than 8mm. The minimal inhibitory concentration was 1:8, such as honeysuckle alcohol extract to Staphylococcus aureus and Subtilis Bacillus. The minimal inhibitory concentration was 1:4 such as honeysuckle alcohol extract to the E. coli. The minimal inhibitory concentration was 1:64 such as wild chrysanthemum alcohol extract to the E. coli. The minimal inhibitory concentration was 1:16 such as wild chrysanthemum alcohol extract to the Staphylococcus aureus. The minimal inhibitory concentration was 1:4 such as wild chrysanthemum alcohol extract to the Subtilis Bacillus.Conclusion The honeysuckle extract has obvious bacteriostatic effect to Staphylococcus aureus and Subtilis Bacillus, but the honeysuckle extract has no inhibitory effect to Escherichia coli and Bacillus. The wild chrysanthemum extract has obvious bacteriostatic effect to Staphylococcus aureus, Escherichia coli and Bacillus, but the honeysuckle extract has no inhibitory effect to Subtilis Bacillus. |
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