| 刘海静,鞠红梅,刘淑娟,王丽,赵向忠,李霞.T细胞亚群偏移与药物流产小鼠子宫出血量相关性研究[J].济宁医学院学报,2013,(2):89-93 |
| T细胞亚群偏移与药物流产小鼠子宫出血量相关性研究 |
| The research of correlation between T-cell subsets and uterine bleeding volume of medical abortion mice |
| 投稿时间：2013-03-15 |
| DOI：10.3969/j.issn.1000-9760.2013.02.004 |
| 中文关键词: 药物流产;子宫出血;T淋巴细胞;Th1/Th2/Th17/Treg |
| 英文关键词: Medical abortion;Uterine bleeding;T lymphocyte;Th1/Th2/Th17/Treg |
| 基金项目:国家自然科学基金项目(No.30901918);山东省科技发展计划(2012GSF11840);山东省博士基金项目(No.BS2009YY041);山东省自然科学基金项目(No.ZR2010HQ066);山东省计生委项目(No.[2009]8);山东省中管局项目(No.2009-173);济南市青年科技明星计划项目(济科计[2012]16号,20120135) |
| 作者 | 单位 | E-mail | | 刘海静 | 济南大学山东省医学科学院医学与生命科学学院, 山东济南 250062
济宁医学院基础学院, 山东济宁 272067 | | | 鞠红梅 | 山东中医药大学附属医院, 山东济南 250011 | | | 刘淑娟 | 山东中医药大学附属医院, 山东济南 250011 | | | 王丽 | 济南大学山东省医学科学院医学与生命科学学院, 山东济南 250062 | | | 赵向忠 | 济南大学山东省医学科学院医学与生命科学学院, 山东济南 250062 | | | 李霞 | 济南大学山东省医学科学院医学与生命科学学院, 山东济南 250062 | lixia979805@sohu.com |
|
| 摘要点击次数: 5200 |
| 全文下载次数: 964 |
| 中文摘要: |
| 目的 探讨药物流产(简称药流)小鼠局部母胎界面T淋巴细胞亚群(Th1/Th2/Th17/Treg)偏移状态与子宫出血量相关性,为减轻药流后子宫出血寻找有效途径。方法 孕鼠30只,随机分为正常妊娠组和米非司酮(RU486)大(1.5 mg/kg/d)、小剂量组(0.88 mg/kg/d),每组10只。改良碱性正铁血红素法检测子宫出血量,流式细胞术检测子宫组织Th1(CD3+CD4+IFNγ+)、Th2(CD3+CD4+IL-4+)、Th17(CD3+CD4+IL-17A+)、Treg(CD4+CD25+Foxp3+)亚群比例。结果 RU486小剂量组小鼠子宫出血量明显大于大剂量组(P<0.05);与正常妊娠组比较,RU486药流小鼠母胎界面Th1、Th17亚群比例升高(P<0.05),Th2、Treg亚群比例降低(P<0.05),Th1/Th2、Th17/Treg比值显著升高(P<0.05);药流小鼠子宫出血量与Th1、Th17亚群比例及Th1/Th2、Th17/Treg比值呈显著负相关(P<0.05),与Th2、Treg亚群比例呈显著正相关(P<0.05)。结论 诱导Th1/Th2/Th17/Treg亚群偏移是RU486药流机制之一,RU486诱导的Th1、Th17偏移状态与子宫出血量间存在密切相关性,靶向诱导Th1、Th17亚群偏移的方法可能成为减轻药流子宫出血的有效途径。 |
| 英文摘要: |
| Objective To investigate the correlation between uterine bleeding volume and shift state of T lymphocyte subsets(Th1/Th2/Th17/Treg)at the maternal-fatal interface of medical abortion mice and to find an effective way to reduce the uterine bleeding during medical abortion.Methods 30 pregnant mice were divided randomly into normal pregnancy group,mifepristone (RU486) high-dose group (1.5 mg/kg/d) and low-dose group (0.88 mg/kg/d),and 10 mice in each group.The uterine bleeding volume of model mice was detected using alkaline hematin photometric method,and the proportion of Th1(CD3+CD4+IFNγ+),Th2(CD3+CD4+IL-4+),Th17(CD3+CD4+IL-17A+) and Treg(CD4+CD25+Foxp3+) of uterine tissue were measured by flow cytometry.Results The uterine bleeding volume of medical abortion mice in RU486-low-dose group was more grater than that of high-dose group (P<0.05).Compared to normal pregnant-mice,the percentage of Th1 and Th17 subsets at the maternal-fetal interface in medical abortion mice increased; the percentage of Th2 and Treg subsets decreased; the ratios of Th1/Th2 and Th17/Treg increased remarkably (P<0.05).The volume of uterine bleeding of medical abortion mice was negatively related to the proportions of Th1 and Th17 cells as well as the ratios of Th1/Th2 cells and Th17/Treg cells (P<0.05),and positively related to the proportions of Th2 and Treg cells (P<0.05).Conclusion Induction of Th1/Th2/Th17/Treg subsets skew is one of the mechanisms during RU486-induced abortion.The state of T cell subsets skew in RU486 medical abortion was closely related to the uterine bleeding volume.Targeting to induce T cell subsets skew may become an effective way to reduce the uterine bleeding during medical abortion. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
