| 张红梅,徐芳芳,卜宪敏,崔文.EGCG对PLA-802细胞中TGF-β1和Smad4的抑制作用[J].济宁医学院学报,2012,(4):251-253 |
| EGCG对PLA-802细胞中TGF-β1和Smad4的抑制作用 |
| Inhibitory effect of EGCG on the expression of TGF-β1 and Smad4 in PLA-802 cells |
| 投稿时间：2012-06-11 |
| DOI：10.3969/j.issn.1000-9760.2012.04.006 |
| 中文关键词: 表没食子儿茶素-3-没食子酸酯;TGF-β1信号通路;PLA-802细胞 |
| 英文关键词: EGCG, TGF-β1 signaling pathway, alveolar rhabdomyosarcoma |
| 基金项目: |
| 作者 | 单位 | E-mail | | 张红梅 | 济宁医学院附属第一人民医院, 山东济宁 272011 | | | 徐芳芳 | 济宁医学院附属第一人民医院, 山东济宁 272011 | | | 卜宪敏 | 济宁医学院附属第一人民医院, 山东济宁 272011 | | | 崔文 | 济宁医学院科研处, 山东济宁 272067 | cuiwenmd@163.com |
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| 中文摘要: |
| 目的 观察表没食子儿茶素-3-没食子酸酯(epigallocatechin-3 gallste,EGCG)对人横纹肌肉瘤细胞株PLA-802细胞内TGF-β1/Smad4mRNA和蛋白水平的表达的影响,探索EGCG抑制横纹肌肉瘤细胞生长的机制。方法 体外培养人横纹肌肉瘤细胞株PLA-802,并用不同浓度的EGCG作用不同的时间,用RT-PCR和Westernblot分别检测细胞内TGF-β1和Smad4的mRNA和蛋白水平的表达。结果 TGF-β1和其下游因子Smad4的mRNA和蛋白水平的表达明显受到EGCG的抑制,且这种抑制作用呈浓度-时间依赖性(P<0.05)。结论 EGCG可能通过抑制TGF-β1信号通路,发挥其抑制PLA-802细胞生长的作用,这或许将为临床治疗横纹肌肉瘤提供新的思路。 |
| 英文摘要: |
| Objective To observe the inhibitory effect of epigallocatechin-3 gallste (EGCG) on the expression of TGF-beta1 and Smad4 in human alveolar rhabdomyosarcoma cell line PLA-802.Methods Human alveolar rhabdomyosarcoma cell line PLA-802 was cultured and treated with EGCG at different concentration and different time points. RT-PCR and western blot were used to detect the expression of TGF-β1 and Smad4 at mRNA and protein levels.Results The expression of of TGF-β1 and its downstream factor-Smad4 at mRNA and protein levels were inhibited by EGCG, and the inhibitory effects of EGCG was in a concentration- and time-dependent manner (P<0.05).Conclusion EGCG probably played an inhibitory effects on PLA-802 cells through inhibiting the activity of TGF-β1 signaling pathway, which would provide an important therapeutic potential for treating human aleolar rhabdomyosarcoma. |
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