| 丁瑞芳,张敏娜,朱军.高效液相色谱法测定可乐中咖啡因含量[J].济宁医学院学报,2012,(2):106-108 |
| 高效液相色谱法测定可乐中咖啡因含量 |
| Determination of caffeine in cola by high performance liquid chromatograph |
| 投稿时间：2012-03-25 |
| DOI：10.3969/j.issn.10009760.2012.02.007 |
| 中文关键词: 高效液相色谱法;可乐;咖啡因 |
| 英文关键词: High performance liquid chromatograph Cola Caffeine |
| 基金项目: |
| 作者 | 单位 | | 丁瑞芳 | 济宁医学院药学院, 山东日照 276826 | | 张敏娜 | 济宁医学院药学院, 山东日照 276826 | | 朱军 | 济宁医学院药学院, 山东日照 276826 |
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| 摘要点击次数: 5624 |
| 全文下载次数: 2009 |
| 中文摘要: |
| 目的 用HPLC测定可乐中咖啡因的含量,建立测定可乐中咖啡因含量的高效液相色谱法的最佳工作条件。方法 色谱柱采用kromasilC18柱(250mm×4.6mm,5μm);流动相:甲醇-水(40:60);流速:1.0ml/min;检测波长为275nm; 进样量20μl;柱温为室温。结果 咖啡因与其它杂质均能达到基线分离,其色谱峰形好。咖啡因的浓度在30~70μg/ml时与峰面积成良好的线性关系(y=27498x+67325,r=0.9994),平均加样回收率为100.1%,方法精密度RSD为1.2%(n=6)。结论 该方法准确、简便、快速,适合可乐中咖啡因的含量测定。 |
| 英文摘要: |
| Objective To determine the determination of caffeine in cola by high performance liquid chromatograph and optimize the HPLC separation conditions. Methods The chromatographic column kromasil C18(250mm×4.6mm, 5μm) and Methanol/H2O (40:60) as mobile phase were used. The flow rate was 1.0ml/min and the detective wavelength was 275nm. Injection volume was 20μl. The column temperature was room temperature. Results Caffeine and other peaks were separated well and the peak shape was presented well. There was a good linearity within the range of 30 to 70μg/ml of caffeine. The average recovery was 101.3%. The RSD was 1.15% (n=6). Conclusion This method is accurate, simple and quick. Therefore it is suitable for the determination of caffeine in cola. |
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