| 刘玉凤,王保国,李会娟,王洪升.芡实多糖的分离纯化及抗氧化作用研究[J].济宁医学院学报,2011,(6):392-394 |
| 芡实多糖的分离纯化及抗氧化作用研究 |
| Study on methods of purifying polysaccharides in semen eurylie and its antioxidant effect |
| 投稿时间：2011-11-05 |
| DOI：10.3969/j.issn.1000-9760.2011.06.004 |
| 中文关键词: 芡实|多糖|大孔树脂|抗氧化 |
| 英文关键词: Semen Eurylie|Polysaccharide|Macroporous resin|Antioxidant |
| 基金项目:济宁医学院2008年青年基金项目 |
| 作者 | 单位 | | 刘玉凤 | 济宁医学院药学院, 山东日照 276826 | | 王保国 | 济宁医学院药学院, 山东日照 276826 | | 李会娟 | 济宁医学院药学院, 山东日照 276826 | | 王洪升 | 济宁医学院药学院, 山东日照 276826 |
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| 中文摘要: |
| 目的 研究芡实多糖的分离纯化方法及其抗氧化作用。方法 用AB-8大孔树脂纯化芡实粗多糖,用Fenton法和邻苯三酚自氧化法测定芡实多糖的体外抗氧化作用。结果 芡实粗多糖经大孔树脂纯化后多糖含量为83.82%;芡实多糖对羟自由基(OH·)和超氧阴离子(O2·)均有一定的清除作用,且其作用强度随芡实多糖浓度增大而增加。结论 AB-8大孔吸附树脂可以用来纯化芡实多糖,芡实多糖具有一定的抗氧化作用,本实验结果为芡实多糖的纯化及进一步开发提供了依据。 |
| 英文摘要: |
| Objective To study the methods of purifying polysaccharides in Semen Eurylie and its antioxidant effect. Methods The polysaccharides were purified by AB-8 macroporous resin.And its antioxidant effect was determined with Fenton method and autoxidation of pyrogallol method. Results The content of polysaccharides was 83.82% after purified by macroporous resin.The purified polysaccharides had some effect on eliminating hydroxyl free radical and superoxide anion and their antioxidant capacity was dose-dependent with concentration. Conclusion AB-8 resin can be used to purify polysaccharides in Semen Eurylie and the purified polysaccharides had a certain antioxidant effect.Practically, the experimental results provide a basis for further development of Semen Eurylie. |
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