| 张越,陈阳,杨元立,李继周,李朝品.法医学组织病理切片STR分型影响因素的比较分析[J].济宁医学院学报,2011,(6):385-388,422 |
| 法医学组织病理切片STR分型影响因素的比较分析 |
| Comparative analysis of influential factors for STR genotyping in forensic pathological sections |
| 投稿时间：2011-11-20 |
| DOI：10.3969/j.issn.1000-9760.2011.06.002 |
| 中文关键词: 法医学|组织病理切片|STR分型 |
| 英文关键词: Forensic genetics|Pathological section|STR genotyping |
| 基金项目:安徽省高校青年教师科研资助计划项目(编号:2007jq1176);安徽省自然科学基金项目(编号:070413088);国家自然科学基金资助项目(编号:C080804) |
| 作者 | 单位 | E-mail | | 张越 | 皖南医学院法医学系, 安徽芜湖 241002 | | | 陈阳 | 皖南医学院法医学系, 安徽芜湖 241002 | | | 杨元立 | 公安部物证鉴定中心, 北京 100038 | | | 李继周 | 公安部物证鉴定中心, 北京 100038 | | | 李朝品 | 皖南医学院法医学系, 安徽芜湖 241002 | cpli001@126.com |
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| 中文摘要: |
| 目的 研究法医学病理切片DNA的基因型,探讨病理切片的组织类型、保存时间与尸源年龄对STR分型的影响。方法 运用16位点Loci-STRs复合荧光标记STR-PCR技术,采用7阶重复拉丁方实验设计,取14例尸检7个年龄段的新鲜组织样本,制作脑、心、肝、肾、脾、肺、肠等7类组织病理切片,将切片随机分组保存7d、14d、30d、90d、180d、360d、720d。用硅珠法提取切片DNA,采用AmpFlSTR·IdentifilerTM 16STR系统进行STR分型。以新鲜尸体DNA样本的基因型为阳性对照,比较分析组织切片DNA的STR基因座检出数。结果 尸源年龄组之间STR基因座检出率的差异不显著(P=0.19)。保存时间分组中,保存30d以内,STR检出数无显著差异(P=0.73),均可准确检出12个以上基因座;保存30d以上,STR基因座检出数与保存时间成负相关;保存360d以上,等位基因脱扣(ADO)与基因座缺失(LOH)显著增高,STR分型不准确。同一保存时间,组织类型之间STR基因座检出率存在显著差异(P<0.01),其中肺组织相对较高。结论 组织切片的保存时间是影响STR分型的主要因素,随着保存时间延长,其STR分型成功率下降;肺组织切片是STR分型较为理想的样本。 |
| 英文摘要: |
| Objective To explore the effects of tissue types, length of pathological sections preserved and death ages on STR genotyping through fluorescent 16 loci multi-STR-PCR detection. Methods Pathological sections were prepared from the brain, heart, liver, lung, spleen, kidney and ileum tissue obtained in 14 cases of fresh autopsy by 7 death ages, and preserved for 7, 14, 30, 90, 180, 360 and 720 days, respectively, in terms of Latin square by double 7-order design.The DNA templates were extracted by silicon bead method, and DNA samples of section were genotyped with AmpFlSTR·IdentifilerTM system in ABI 310 Genetic Analyzer.Additional DNA was extracted from the fresh autopsies as positive controls. Results Death age group showed no significant difference in detection of STR loci(P>0.05).No difference was found in the sections preserved for 30 days concerning STR loci(P>0.05), though 12 loci were detected on STR that was negatively correlated with the profiling success number and length of storage.Allele dropout(ADO) and loss of heterozygosity(LOH) occurred more frequently in the sections maintained beyond 360 days, which commonly led to faint STR genotyping.Yet, different tissues maintained in the same length revealed significant difference with the accurately detected STR loci, which remained the highest in the lung section(P<0.01). Conclusion The length of sections saved tends to affect the STR genotyping and accurate detection of it in pathological sections with time extension.Apart from that, pulmonary tissue section appears to be favorable samples for STR genotyping in individual DNA identification. |
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