| 路海,胡景霞,陈京,白波.吗啡持续刺激对小鼠树突状细胞发育及分泌IL-12的影响[J].济宁医学院学报,2011,(4):229-231,235 |
| 吗啡持续刺激对小鼠树突状细胞发育及分泌IL-12的影响 |
| Effects of sustained morphine stimulation on dendritic cell development and IL-12 production |
| 投稿时间：2011-06-21 |
| DOI：10.3969/j.issn.1000-9760.2011.04.001 |
| 中文关键词: 吗啡;树突状细胞;阿片受体;白介素-12 |
| 英文关键词: Morphine;Dendritic cell;Opioid receptor;IL-12 |
| 基金项目:国家自然科学基金项目(编号:30971081、30870932、81070961);山东省自然基金资助项目(编号:ZR2009DZ004) |
| 作者 | 单位 | E-mail | | 路海 | 济宁医学院神经生物学研究所, 山东济宁 272067 | | | 胡景霞 | 泰山医学院, 山东泰安 271016 | | | 陈京 | 济宁医学院神经生物学研究所, 山东济宁 272067 | | | 白波 | 济宁医学院神经生物学研究所, 山东济宁 272067 | bbai@mail.jnmc.edu.cn |
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| 中文摘要: |
| 目的 吗啡持续刺激对C57 BL/6小鼠骨髓来源的树突状细胞成熟的影响。方法 体外培养C57 BL/6小鼠骨髓来源的树突状细胞,培养过程中给予吗啡刺激,观察细胞形态的变化;应用酶联免疫吸附法检测树突状细胞分泌细胞因子IL-12水平的变化。结果 吗啡干预各组树突状细胞中细胞因子IL-12分泌水平较对照组显著升高。结论 吗啡持续刺激能够促进小鼠树突状细胞的成熟。 |
| 英文摘要: |
| Objective To investigate the effects of sustained morphine stimulation on the bone marrow-derived dendritic cell（DC） maturation.Methods The primary C57BL/6 mouse bone marrow-derived dendritic cells（4-6weeks） were cultured in vitro.In course of cell culture morphine was added at the same time.The cell supernatants were collected at 7 days and interleukin-12（IL-12） levels were detected by enzyme-linked immunosorbent assay（ELISA）.Results The results showed that the secretion of IL-12 in LPS＋morphine groups was significantly increased compared with blank control group and LPS control group（P<0.01）.But there was no difference between the LPS＋morphine groups（P>0.05）.Conclusion Sustained morphine stimulation on the mouse bone marrow-derived dendritic cells can promote dendritic cells to mature. |
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