| 窦姗姗,邓景惕,高文明.辛伐他汀对脂多糖刺激人脐静脉内皮细胞Tribbles表达的影响[J].济宁医学院学报,2011,(3):156-159 |
| 辛伐他汀对脂多糖刺激人脐静脉内皮细胞Tribbles表达的影响 |
| The effect of simvastatin on the expression of tribbles in HUVECs with LPS treatment |
| 投稿时间：2011-04-17 |
| DOI：10.3969/j.issn.1000-9760.2010.06.002 |
| 中文关键词: 辛伐他汀|脂多糖|Tribbles|肿瘤坏死因子|人脐静脉内皮细胞 |
| 英文关键词: Simvastatin|Lipopolysaccharides|Tribbles|TNF-α|Human umbilical vein endothelial cells |
| 基金项目: |
| 作者 | 单位 | E-mail | | 窦姗姗 | 济宁医学院基础与法医学院, 山东济宁 272067 | | | 邓景惕 | 山东大学医学院, 山东济南 250012 | | | 高文明 | 济宁医学院基础与法医学院, 山东济宁 272067 | ppc32960@yahoo.com.cn |
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| 中文摘要: |
| 目的 研究脂多糖(LPS)对人脐静脉内皮细胞(HUVECs)肿瘤坏死因子(TNF-α)和TribblesmRNA表达变化及辛伐他汀对其表达的影响。方法 实验分为12 h组和24 h组,各组又分为空白对照组、脂多糖(100 ng/ml)组、辛伐他汀(5μg/ml)组、辛伐他汀(5μg/ml)和脂多糖(100 ng/ml)共作用组,用实时荧光定量PCR测定LPS刺激后12 h和24 h HUVECsTNF-α和Trb1、Trb2及Trb3的mRNA表达变化。结果 与对照组相比,脂多糖组TNF-α、Trb1及Trb3的mRNA表达均明显增加(P均<0.05);与脂多糖组相比,辛伐他汀和脂多糖共作用组TNF-α、Trb1及Trb3的mRNA表达均明显降低(P均<0.05)。结论 辛伐他汀可抑制HU-VECs的TNF-α、Trb1及Trb3的表达。 |
| 英文摘要: |
| Objective To explore the expression of TNF-α and Tribbles mRNA in HUVECs with LPS treatment and the effect of simvastatin on them.Methods HUVECs were cultured and divided into the following experimental groups: control group, LPS group(100ng/mL), simvastatin group(5μg/mL), and LPS(100ng/mL)+simvastatin(5μg/mL) group for 12h and 24h respectively.The expression of TNF-α, Trb1, Trb2, Trb3 mRNA in HUVECs with LPS treatment after 12h and 24h was measured using real-time quantitative PCR.Results Compared with the control groups, the expression of TNF-α, Trb1 and Trb3 mRNA was significantly increased in LPS groups(P<0.05).However, the expression of TNF-α, Trb1and Trb3 mRNA was statistically decreased in LPS and simvastatin groups compared with LPS groups, (P<0.05).Conclusion Simvastatin could inhibit the expression of TNF-α, Trb1, Trb3 in HUVECs induced by LPS. |
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