| 何雪琦,史志涛,鲍扬漪.1,25-二羟维生素D3联合塞莱昔布对乳腺癌细胞株HS578T增殖的影响[J].济宁医学院学报,2011,(1):17-19 |
| 1,25-二羟维生素D3联合塞莱昔布对乳腺癌细胞株HS578T增殖的影响 |
| The effect of combined treatment of 1,25(OH)2D3] and Celecoxib on the growth of breast cancer cell line HS578T |
| 投稿时间：2010-11-30 |
| DOI：10.3969/j.issn.1000-9760.2011.01.006 |
| 中文关键词: 1,25-二羟维生素D3|塞莱昔布|乳腺肿瘤|细胞增殖 |
| 英文关键词: 1,25-dihydroxyvitaminD3|Celecoxib|Breast cancer|Cell proliferation |
| 基金项目:合肥市科技局重点立项项目(编号:合科2007第15号) |
| 作者 | 单位 | E-mail | | 何雪琦 | 济宁医学院临床学院, 山东济宁 272067 | | | 史志涛 | 济宁医学院附属医院, 济宁 272029 | | | 鲍扬漪 | 合肥市第一人民医院, 安徽合肥 230000 | baoyyi@yahoo.com.cn |
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| 中文摘要: |
| 目的 研究1,25-二羟维生素D3[1,25(OH)2D3]联合塞莱昔布对乳腺癌细胞株HS578T生长的影响。方法 采用四唑氮蓝比色(MTT)法检测细胞增殖,免疫组化法检测Ki-67表达变化。结果 10-7mol/L、10-8mol/L的1,25(OH)2D3]及塞莱昔布(50μmol/L)均可抑制乳腺癌细胞增殖,呈时间与剂量依赖性。免疫组化结果显示各药物处理组细胞Ki-67表达率与对照组比较明显减低。结论 1,25(OH)2D3对HS578T细胞的体外增殖具有抑制作用,联合塞莱昔布具有协同作用。 |
| 英文摘要: |
| Objective To study the effects of combined 1,25-dihydroxyvitamin D3[1,25(OH)2D3] and Celecoxib on the proliferation in breast cancer cell line HS578T.Methods We compared cell numbers by using MTT method, The expression of Ki-67 was detected by immunohistochemical method.Results Incubation with 10-7mol/L and 10-8mol/L1,25(OH)2D3], Hs578T cells exhibited significant growth inhibition, The immunohistochemical staining showed that the expression rate of Ki-67 protein was down-regulated in any treatemnt group compared to normal group.Conclusion 1,25(OH)2D3 and Celecoxib could inhibit the proliferation and induce apoptosis of breast cancer cell line HS578T. |
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