文章摘要
陈思颖,苏志恒.桂枝配方颗粒抗氧化活性的快速测定[J].济宁医学院学报,2024,47(2):77-82
桂枝配方颗粒抗氧化活性的快速测定
Rapid determination of antioxidant activity of Guizhi formula granules based on near infrared spectroscopy
投稿时间:2023-12-25  
DOI:10.3969/j.issn.1000-9760.2024.02.001
中文关键词: 近红外光谱;桂枝配方颗粒;抗氧化活性
英文关键词: Near infrared spectroscopy;Guizhi formula granule;Antioxidant activity
基金项目:广西创新驱动发展专项基金项目(2022JBGS042)
作者单位E-mail
陈思颖 广西医科大学药学院, 南宁 530021  
苏志恒 广西医科大学药学院, 南宁 530021 suzhiheng915@126.com 
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中文摘要:
      目的 建立近红外光谱法对桂枝配方颗粒进行抗氧化活性的快速测定。方法 采用便携式近红外光谱仪,采集30批桂枝配方颗粒样品的漫反射近红外光谱数据,使用不同光谱预处理方法对原始光谱进行光谱预处理,采用组合区间偏最小二乘算法(partial least squares regression,PLS)和支持向量回归算法(support vector regression,SVR)对建模波数区间进行优化,并以1,1-二苯基-2-三硝基苯肼(2,2-Diphenyl-1-picrylhydrazyl,DPPH)、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(2,2’-Azinobis-(3-ethylbenzthiazoline-6-sulphonate),ABTS)、铁离子抗氧化能力(ferric ion reducing antioxidant power,FRAP)法为参考方法,建立桂枝配方颗粒的抗氧化活性定量分析模型。结果 近红外光谱法可有效预测不同批次桂枝配方颗粒的抗氧化活性。DPPH方法测定桂枝配方颗粒最佳PLS模型的预测相关系数(predictive correlation coefficient,Rp)为0.769,校正相关系数(corrected correlation coefficient,Rc)为0.795;ABTS方法最佳SVR模型的Rp值为0.928,Rc值为0.992;FARP方法最佳SVR模型的Rp值为0.573,Rc值为0.926。结论 近红外光谱可以应用于不同批次桂枝配方颗粒抗氧化活性的快速预测,对其他中药配方颗粒生物学活性的快速评价具有一定的参考意义。
英文摘要:
      Objective To establish a near infrared spectroscopy method for the rapid determination of antioxidant activity of Guizhi formula granules.Methods A portable near-infrared spectrometer was used to collect diffuse near-infrared spectral data of 30 batches of samples of Guizhe formula particles.The original spectra were preprocessed by different spectral pretreatment methods.PLS and support vector regression (SVR) were used to optimize the wave number interval,and DPPH,ABTS and FRAP were used as reference methods to establish a quantitative analysis model of antioxidant activity of Guizhi formula granules.Results The near-infrared spectroscopy method can effectively predict the antioxidant activity of different batches of Guizhi formula particles.The predictive correlation coefficient (Rp) and corrected correlation coefficient (Rc) of DPPH method for PLS model were 0.769 and 0.795 respectively.The Rp value and Rc value of the SVR model corresponding to ABTS method are 0.928 and 0.992.The Rp value and Rc value of the SVR model corresponding to FARp method are 0.573 and 0.926.Conclusion The near infrared spectroscopy can be used to predict the antioxidant activity of different batches of Guizhe formula granules,and has certain reference significance for the rapid evaluation of biological activity of other Chinese medicine formula granules.
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