| 窦姗姗,高丽颖,林馨怡,孟尧,董康,程葆华.Apelin-36对鱼藤酮诱导的SH-SY5Y细胞凋亡及线粒体功能的影响[J].济宁医学院学报,2022,45(4):234-238 |
| Apelin-36对鱼藤酮诱导的SH-SY5Y细胞凋亡及线粒体功能的影响 |
| Effects of apelin-36 on the apoptosis and mitochondrial dysfunction of SH-SY5Y cells induced by rotenone |
| 投稿时间：2022-05-11 |
| DOI：10.3969/j.issn.1000-9760.2022.04.002 |
| 中文关键词: Apelin-36;鱼藤酮;帕金森病;线粒体损伤;凋亡 |
| 英文关键词: Apelin-36;Rotenone;Parkinson's disease;Mitochondrial damage;Apoptosis |
| 基金项目:济宁医学院教师扶持基金(JYFC2018KJ003);大学生创新训练项目(CX2019205) |
| 作者 | 单位 | E-mail | | 窦姗姗 | 济宁医学院基础医学院, 济宁 272067 | | | 高丽颖 | 济宁医学院基础医学院, 济宁 272067 | | | 林馨怡 | 济宁医学院基础医学院, 济宁 272067 | | | 孟尧 | 济宁医学院基础医学院, 济宁 272067 | | | 董康 | 济宁医学院基础医学院, 济宁 272067 | | | 程葆华 | 济宁医学院基础医学院, 济宁 272067 | chengbh1979@163.com |
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| 中文摘要: |
| 目的 探讨apelin-36对鱼藤酮诱导的SH-SY5Y细胞凋亡及线粒体功能的影响。方法 培养SH-SY5Y细胞,随机分为对照组、apelin-36组、鱼藤酮组和apelin-36+鱼藤酮组。通过鱼藤酮处理SH-SY5Y细胞建立帕金森病(Parkinson's disease,PD)体外模型,在给予apelin-36预处理后,通过CCK-8观察细胞活力,Hoechst 33342检测细胞凋亡,Western blotting检测Bax、bcl-2、α-synuclein的表达,试剂盒检测线粒体复合物I (complex I)的表达和ADP/ATP的比值来观察线粒体功能。结果 与对照组相比,鱼藤酮组细胞活力下降,细胞凋亡增加,α-synuclein的表达增加,bcl-2/Bax比值下降,complex I表达降低,ADP/ATP比率升高(均P<0.05);而给予apelin-36预处理后,与鱼藤酮组相比,apelin-36+鱼藤酮组细胞活力上升,细胞凋亡降低,α-synuclein的表达减少,bcl-2/Bax比值增高,complex I表达升高,ADP/ATP比率降低(均P<0.05)。。结论 Apelin-36对鱼藤酮引起的SH-SY5Y细胞凋亡具有抑制作用,可减轻鱼藤酮所致的SH-SY5Y细胞线粒体功能紊乱,起到神经保护作用。 |
| 英文摘要: |
| Objective To investigate the effects of apelin-36 on rotenone-induced apoptosis and mitochondrial dysfunction of SH-SY5Y cells.Methods SH-SY5Y cells were cultured and randomly divided into control group,apelin-36 group,rotenone group and apelin-36 + rotenone group.The vitro model of Parkinson's disease (PD) was established by treating SH-SY5Y cells with rotenone.After apelin-36 pretreatment,the cell viability was measured by CCK-8 assay.The apoptosis was measured by Hoechst 33342 staining.The expression of Bax,bcl-2 and α-synuclein was measured by western blotting.Mitochondrial function was detected by complex I and ADP/ATP ratio.Results Compared with the control group,the cell viability was decreased and apoptosis was increased in rotenone group.And α-synuclein expression was increased significantly in SH-SY5Y cells with rotenone treatment.Bcl-2/Bax ratio was decreased due to rotenone exposure.Complex I expression was decreased and ADP/ATP ratio was increased after rotenone treatment in SH-SY5Y cells.However,apelin-36 pretreatment significantly inhibited rotenone neurotoxicity including increasing cell viability and decreasing apoptosis of SH-SY5Y cells.Compared with rotenone group,α-synuclein expression was decreased and bcl-2/Bax ratio was increased significantly.And complex I expression was increased and ADP/ATP ratio was decreased significantly due to apelin-36 prereatment.Conclusion Apelin-36 inhibited rotenone-induced the apoptosis of SH-SY5Y cells and alleviated rotenone-induced mitochondrial dysfunction,which played a neuroprotective role against rotenone neurotoxicity. |
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