| 李文博,史宏超,林英豪,李淼,李姝,刘晓阳,徐静,徐香玉.球形纳米金与牛血清白蛋白的相互作用[J].济宁医学院学报,2021,44(5):305-309,314 |
| 球形纳米金与牛血清白蛋白的相互作用 |
| The interaction of spherical gold nanoparticles with bovine serum albumin |
| 投稿时间：2021-05-26 |
| DOI：10.3969/j.issn.1000-9760.2021.05.001 |
| 中文关键词: 球形纳米金;牛血清白蛋白;荧光光谱;荧光猝灭;热力学参数;圆二色谱 |
| 英文关键词: Spherical gold nanoparticles;Bovine serum albumin;Fluorescence spectra;Fluorescence quenching;Thermodynamic parameters;Circular dichroism |
| 基金项目:济宁医学院国家自然科学基金培育基金项目（JYP2018KJ17）；济宁医学院大学生创新训练计划项目（cx2020100） |
| 作者 | 单位 | E-mail | | 李文博 | 济宁医学院基础医学院, 济宁 272067 | | | 史宏超 | 济宁医学院基础医学院, 济宁 272067 | | | 林英豪 | 济宁医学院基础医学院, 济宁 272067 | | | 李淼 | 济宁医学院基础医学院, 济宁 272067 | | | 李姝 | 济宁医学院基础医学院, 济宁 272067 | | | 刘晓阳 | 济宁医学院基础医学院, 济宁 272067 | | | 徐静 | 济宁医学院附属医院, 济宁 272029 | 873693629@qq.com | | 徐香玉 | 济宁医学院基础医学院, 济宁 272067 | xuxiangyu1212@163.com |
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| 中文摘要: |
| 目的 探索球形纳米金与牛血清白蛋白（BSA）之间的相互作用，获得相应的热力学参数以及猝灭机理。方法 利用紫外可见吸收光谱法和荧光光谱法对反应体系进行光谱学实验。利用圆二色谱法对BSA的分子结构进行研究。结果 随着球形纳米金溶液浓度的增加，反应体系的紫外吸收峰强度增大，而荧光强度却明显猝灭且荧光峰位由278 nm蓝移到了268 nm。3种温度（298K，303K，308K）下的Kq值分别为3.513×1010、4.033×1010和4.787×1010 L·mol-1·s-1，均大于2.0×1010L·mol-1·s-1，由此可以得知球形纳米金和BSA的相互作用是静态猝灭过程。由ΔG<0，ΔH<0和ΔS<0，可知两者之间的相互作用是自发进行的，且主要作用力是氢键和范德华力。球形纳米金的存在影响了BSA分子的二级结构，使其α-螺旋结构含量由56.9%降低到32.2%。结论 本研究获得了球形纳米金与BSA相互作用的热力学参数以及蛋白质二级结构含量的变化特征，为金纳米材料在生物医学领域的应用提供基础理论依据。 |
| 英文摘要: |
| Objective Study the interaction between spherical gold nanoparticles and bovine serum albumin(BSA) to obtain the corresponding thermodynamic parameters and quenching mechanism.Methods The interaction between spherical gold nanoparticles and BSA was studied by UV-visible absorption spectroscopy(UV-vis) and fluorescence spectroscopy(FL).The molecular structure of BSA was studied by circular dichroism(CD).Results With the concentration of the spherical gold nanoparticles solution increases, the absorbance intensity of the mixed solution increases.However, the fluorescence intensities obviously quenched and the fluorescence peak position is blue-shifted from 278 nm to 268 nm.The Kq values at the three temperatures(298K, 303K, 308K) are 3.513×1010, 4.033×1010 and 4.787×1010 L·mol-1·s-1, respectively.The values are all greater than 2.0×1010L·mol-1·s-1, which shows that the interaction between spherical gold nanoparticles and BSA is a static quenching process.ΔG<0, ΔH<0 and ΔS<0 are obtained from the variable temperature fluorescence experiment indicating that the interaction is spontaneous, and the main force is hydrogen bonds and Van der Waals forces.The experimental results of circular dichroism(CD) show that the presence of spherical gold nanoparticles affects the secondary structure of BSA molecules, reducing the content of α-helical structure from 56.9% to 32.2%.Conclusion The thermodynamic parameters of the interaction between spherical gold nanoparticles and BSA and the changes in protein secondary structure content have been obtained in this study, which provides a basic theoretical foundation for the application of gold nanomaterials in the field of biomedicine. |
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