随蓓蓓,李华舜,孟晓,孙秀玲,刘莉.三七总皂苷对人乳腺癌MDA-MB-231细胞凋亡的影响[J].济宁医学院学报,2021,44(2):77-79,83 |
三七总皂苷对人乳腺癌MDA-MB-231细胞凋亡的影响 |
Apoptosis of human breast cancer cell line MDA-MB-231 by Panax notoginseng saponins |
投稿时间:2020-11-20 |
DOI:10.3969/j.issn.1000-9760.2021.02.001 |
中文关键词: 三七总皂苷;细胞活性;凋亡 |
英文关键词: Panax notoginseng saponins;Cellular activity;Apoptosis |
基金项目:国家级大学生创新项目(201610443086) |
作者 | 单位 | 随蓓蓓 | 济宁医学院生物科学学院, 日照 276826 | 李华舜 | 济宁医学院生物科学学院, 日照 276826 | 孟晓 | 济宁医学院生物科学学院, 日照 276826 | 孙秀玲 | 济宁医学院生物科学学院, 日照 276826 | 刘莉 | 济宁医学院生物科学学院, 日照 276826 |
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中文摘要: |
目的 探究三七总皂苷对人乳腺癌MDA-MB-231细胞凋亡的影响。方法 三七总皂苷作用于人乳腺癌MDA-MB-231细胞作为处理组,同时设置空白对照组; MTT法检测细胞活性变化,光镜观察检测细胞形态学变化,凋亡试剂盒检测细胞凋亡情况,casepase 3试剂盒检测casepase 3的酶活性情况。结果 与空白对照组相比,0.5、1.0和2.0mg/ml三七总皂苷组人乳腺癌MDA-MB-231细胞活性逐渐降低(P<0.05),呈时间和浓度依赖性;光镜观察下1.0、2.0mg/ml三七总皂苷组产生了凋亡小体;荧光观察下1.0、2.0mg/ml三七总皂苷组出现了核固缩现象;与空白对照组相比,0.5、1.0和2.0mg/ml三七总皂苷组人乳腺癌MDA-MB-231细胞casepase3的酶活性明显增加,差异具有统计学意义(P<0.05)。结论 三七总皂苷可降低人乳腺癌MDA-MB-231细胞的活性,具有促凋亡作用。 |
英文摘要: |
Objective To explore the extraction of Panax notoginseng saponins(PNS) from Panax notoginseng and the effect on human breast cancer cell line MDA-MB-231.Methods The total saponin of hepaescin was used as treatment group on human breast cancer cell MDA-MB-231.The blank control group was set up at the same time.MTT method was used to detect the changes of cell activity;the morphological changes of cells were detected by light microscope;apoptosis was detected by apoptosis kit;the expression of casepase-3 was detected by kit.Results Compared with the blank control group,the activity of MDA-MB-231 cells in 0.5,1.0,2.0mg/ml groups decreased gradually in a time and concentration dependent manner;apoptotic bodies were produced in 1.0 and 2.0mg/ml PNS groups;karyopyknosis was observed in 1.0 and 2.0mg/ml PNS groups;compared with the blank control group,the enzyme activity of MDA-MB-231 casepase-3 in 0.5,1.0 and 2.0mg/ml PNS groups was significantly increased,and the difference was statistically significant (P<0.05).Conclusion Panaxnotoginsengsaponins can reduce the activity of MDA-MB-231 cells and promote apoptosis. |
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