| 刘超,张腾元,朱日明,高鑫芯,高慧婕.槐耳清膏对LPS致小鼠炎症反应的调节作用[J].济宁医学院学报,2020,43(6):389-392 |
| 槐耳清膏对LPS致小鼠炎症反应的调节作用 |
| Experimental study on anti-inflammatory activity of Huaier cream in mice with LPS induced inflammation |
| 投稿时间：2020-07-09 |
| DOI：10.3969/j.issn.1000-9760.2020.06.003 |
| 中文关键词: 槐耳清膏;LPS;炎症;IL-6;TNF-α |
| 英文关键词: Huaier cream;LPS;Inflammation;TNF-α;IL-6 |
| 基金项目:山东省自然科学基金（ZR2016HL22）；山东省高等学校科技计划（J18KA297）；国家大学生创新训练计划项目（201810443020） |
| 作者 | 单位 | E-mail | | 刘超 | 济宁医学院药学院, 日照 276826 | | | 张腾元 | 济宁医学院药学院, 日照 276826 | | | 朱日明 | 济宁医学院药学院, 日照 276826 | | | 高鑫芯 | 济宁医学院药学院, 日照 276826 | | | 高慧婕 | 济宁医学院药学院, 日照 276826 | mianyigao@163.com |
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| 中文摘要: |
| 目的 制备脂多糖（LPS）致炎小鼠模型，观察槐耳清膏对小鼠炎症反应的调节作用。方法 ICR小鼠随机分为正常对照组、LPS组、槐耳清膏低剂量组（4mg/kg）、中剂量组（6mg/kg）和高剂量组（8mg/kg），每组10只。6d后测定小鼠脾脏指数，HE染色观察小鼠肺脏组织的病理变化；ELISA检测小鼠血清IL-6、TNF-α的表达，RT-qPCR检测小鼠脾脏IL-6、TNF-α mRNA的变化。结果 与正常对照组相比，LPS组小鼠的脾脏指数（5.09±0.80）和IL-6[（52.22±8.41）pg/ml]、TNF-α[（56.40±5.97）pg/ml]、IL-6 mRNA（3.16±0.89）和TNF-α mRNA（3.02±0.84）表达水平均显著升高，小鼠肺部炎症反应增加，说明LPS致炎症模型制备成功；与LPS组相比，高剂量槐耳清膏干预后的脾脏指数（3.62±0.69）和IL-6[（37.65±6.35）pg/ml]、TNF-α[（34.94±5.12）pg/ml]、IL-6 mRNA（1.32±0.15）和TNF-α mRNA（1.29±0.19）均有显著的降低（均P<0.05）。结论 槐耳清膏可抵抗由LPS所致小鼠炎症，降低炎症性细胞因子的表达，有良好的抗炎效果。 |
| 英文摘要: |
| Objective To observe the protective effect of Huaier cream in mouse with lipopolysaccharide(LPS) induced inflammation and to explore the mechanism of anti-inflammatory activity of Huaier cream at the level of cytokines.Methods ICR mice were randomly divided into control group, LPS group, Huaier cream high, middle and low dose(8, 6 and 4 mg/kg) group.After 6 days, the spleen index were analyzed and the inflammatory changes of lung tissues in each group were observed by hematoxylin and eosin(HE) staining techniques.The expression of TNF-α and IL-6 in peripheral blood was detected by ELISA.RT-qPCR was used to detect the changes of TNF-α and IL-6 mRNA expression in mouse spleen.Results Compared with control group, LPS-treated mice showed significant increase in the spleen index(5.09±0.80), IL-6[(52.22±8.41)pg/ml], TNF-α[(56.40±5.97)pg/ml], IL-6 mRNA(3.16±0.89) and TNF-α mRNA(3.02±0.84), and inflammatory changes of lung tissues in mouse.Compared with LPS model group, the Huaier cream significantly decreased the spleen index(3.62±0.69), IL-6[(37.65±6.35)pg/ml], TNF-α[(34.94±5.12)pg/ml], IL-6 mRNA(1.32±0.15) and TNF-α mRNA(1.29±0.19), and inflammatory changes of lung tissues in mouse.Conclusion Huaier cream exhibits good anti-inflammatory effect in mouse with LPS induced inflammation. |
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