赛春梅,贾玲玉,赵爽,张国印,高峰,张方鹏.二仙膏总皂苷总多糖的含量测定[J].济宁医学院学报,2020,43(2):83-86 |
二仙膏总皂苷总多糖的含量测定 |
Determination of total saponins and polysaccharides in Erxiangao |
投稿时间:2019-12-03 |
DOI:10.3969/j.issn.1000-9760.2020.02.002 |
中文关键词: 二仙膏;总皂苷;总多糖;紫外可见分光光度法 |
英文关键词: Erxiangao;Total saponins;Total polysaccharides;UV-visible spectrophotometry |
基金项目:中国博士后科学基金面上资助项目(2018M642604) |
作者 | 单位 | 赛春梅 | 济宁医学院药学院, 日照 276826 | 贾玲玉 | 济宁医学院药学院, 日照 276826 | 赵爽 | 济宁医学院药学院, 日照 276826 | 张国印 | 山东广育堂国药有限公司, 济宁 272071 | 高峰 | 山东广育堂国药有限公司, 济宁 272071 | 张方鹏 | 山东广育堂国药有限公司, 济宁 272071 |
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中文摘要: |
目的 建立二仙膏总皂苷、总多糖含量的测定方法。方法 分别以齐墩果酸和无水葡萄糖为对照品,采用紫外可见分光光度法测定二仙膏总皂苷、总多糖的含量。结果 总皂苷测定的最大吸收波长为540nm,齐墩果酸在浓度范围0.021~0.070mg/ml,吸光度与浓度呈良好的线性关系,回归方程为y=11.182x-0.0629(r=0.9999),平均加样回收率为96.77%,RSD为1.61%,二仙膏中总皂苷平均含量8.62mg/g。总多糖测定的最大吸收波长620nm,无水葡萄糖在浓度范围0.024~0.120mg/ml,吸光度与浓度呈良好的线性关系,回归方程为y=6.8708x+0.0231(r=0.9999),平均加样回收率为101.83%,RSD为0.92%,总多糖平均含量41.43%。结论 该方法简便、快速、准确,重复性好,稳定可靠,可作为二仙膏总皂苷、总多糖含量的测定方法,为二仙膏的质量控制和下一步二仙膏的物质基础研究奠定基础。 |
英文摘要: |
Objective To develop a method for determinating total saponins and polysaccharides in Erxiangao.Methods Using oleanolic acid and glucosumanhydricumas reference substances respectively,the content total saponins and polysaccharides in Erxiangao were determined by UV-visible spectrophotometry.Results The detection maximum absorption wavelength of total saponins was 540nm.Within the concentration range of 0.021~0.070mg/ml,the absorbance had a good linear relationship with the concentration of oleanolic acid,and the regression equation was y=11.182x-0.0629(r=0.9999).The average recovery was 96.77%,and RSD was 1.61%.The average content of total saponins in Erxiangao was 8.62mg/g.The detection maximum absorption wavelength of total polysaccharides was 620nm.Within the concentration range of 0.024~0.120mg/ml,the absorbance had a good linear relationship with the concentration of glucosumanhydricum and the regression equation was y=6.8708x+0.0231(r=0.9999).The average recovery was 101.83%,and RSD was 0.92%.The average content of total polysaccharides in Erxiangao was 41.43%.Conclusion The method was simple,rapid,accurate,stable and reliable,which could be used for the determination of total saponins and polysaccharides in Erxiangao.It had laid a foundation for the quality control and further study on the material basis of Erxiangao. |
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