| 郑培兵,王伟芳,张绪利,郝延磊,冯勋刚.布洛芬对小鼠运动神经元轴索生长的影响及分子机制[J].济宁医学院学报,2019,42(2):126-130 |
| 布洛芬对小鼠运动神经元轴索生长的影响及分子机制 |
| Effects of ibuprofen on axonal growth of mouse motor neuron and its possible mechanism |
| 投稿时间：2018-11-22 |
| DOI：10.3969/j.issn.1000-9760.2019.02.012 |
| 中文关键词: 运动神经元;布洛芬;轴索生长 |
| 英文关键词: Motor neuron;Ibuprofen;Axonal growth |
| 基金项目:国家自然科学基金（81771360）；山东省自然科学基金（ZR2017LH034） |
| 作者 | 单位 | E-mail | | 郑培兵 | 济宁医学院临床医学院, 济宁 272013 | | | 王伟芳 | 济宁医学院附属医院, 济宁 272029 | | | 张绪利 | 济宁医学院临床医学院, 济宁 272013 | | | 郝延磊 | 济宁医学院附属医院, 济宁 272029 | | | 冯勋刚 | 济宁医学院附属医院, 济宁 272029 | jyfyfxg@163.com |
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| 中文摘要: |
| 目的 观察布洛芬对小鼠运动神经元轴索生长的影响并探讨其可能的分子机制。方法 利用密度梯度离心法分离孕13.5d的C57BL/6小鼠胚胎脊髓前角运动神经元，随机分为布洛芬组、神经生长因子（NGF）组和对照组，布洛芬组、NGF组分别给予400μmol/L的布洛芬、100ng/ml的NGF，以无处理的对照组作为对照。培养48h后以轴索生长测定观察布洛芬对运动神经元轴索生长的影响，收集不同处理的运动神经元测定Rho激酶（ROCK）活性，荧光定量PCR检测MAPK信号分子（ERK，p38，JNK）的表达。结果 与对照组相比，布洛芬组的神经元轴索长度偏长（P<0.05），ROCK活性显著偏低且JNK mRNA的相对表达量显著偏高（P<0.05）；与NGF组相比，布洛芬组的神经元轴索长度及其ROCK活性无差异（均P>0.05），但JNK mRNA的相对表达量高于NGF组（P<0.05）。3组间ERK和p38的表达均无显著性差异（P>0.05）。结论 布洛芬可能通过上调JNK的表达而促进运动神经元轴索的生长。 |
| 英文摘要: |
| Objective To investigate the effects of ibuprofen on axonal growth of motor neuron and explore its possible mechanism.Methods The primary motor neuron was prepared from the embryonic spinal cord of C57BL/6 mouse on day 13.5 using the density gradient centrifugation and randomly divided into three groups:the ibuprofen group,nerve growth factor (NGF) group and control group.The ibuprofen group and the NGF group were treated with 400μmol/L of ibuprofen and 100 ng/ml of NGF,respectively.The motor neuron without treatment was used as the control group.After 48 hours,axonal growth assay was performed to investigate the effects of ibuprofen on axonal growth of motor neuron.The neurons with different treatment were collected for Rho kinase activity and Real-time PCR was performed to investigate the relative expression of MAPK signaling pathway molecules (ERK,p38,JNK).Results Compared with the control groups,the axonal length of the ibuprofen groups was longer (P>0.05),and the Rho kinase activity was significantly lower than controls (P>0.05).The PCR analysis showed that there was higher relative expression of JNK mRNA in ibuprofen groups (P>0.05).Compared with the NGF groups,there was no difference in neuronal axonal length and ROCK activity in the ibuprofen groups(P>0.05).However,the relative expression of JNK mRNA was higher than that of the NGF-treated (P<0.05).There was no difference in relative expression of both ERK and p38 among the three groups(P>0.05).Conclusion Ibuprofen may promote the axonal growth of motor neuron by upregulating the expression of JNK and inhibiting the Rho kinase activity. |
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