吕美,王利涛,许欣欣,韩冠媛,王豪帅,于爽.HPLC法同时检测苦参碱杀虫剂中生物碱含量[J].济宁医学院学报,2017,40(4):268-270 |
HPLC法同时检测苦参碱杀虫剂中生物碱含量 |
Determination of the content of alkaloids from matrine pesticides by HPLC |
投稿时间:2017-07-05 |
DOI:10.3969/j.issn.1000-9760.2017.04.008 |
中文关键词: 苦参碱杀虫剂;生物碱;高效液相色谱法;含量测定 |
英文关键词: Matrine pesticides;Alkaloids;HPLC method;Content determination |
基金项目:国家自然科学基金项目(21405162) |
作者 | 单位 | E-mail | 吕美 | 济宁医学院药学院, 日照 276826 | | 王利涛 | 济宁医学院药学院, 日照 276826 | wanglilvtao@163.com | 许欣欣 | 济宁医学院药学院, 日照 276826 | | 韩冠媛 | 济宁医学院药学院, 日照 276826 | | 王豪帅 | 济宁医学院药学院, 日照 276826 | | 于爽 | 济宁医学院药学院, 日照 276826 | |
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中文摘要: |
目的 建立高效液相色谱法同时测定苦参碱杀虫剂中苦参碱、氧化苦参碱、槐定碱和槐果碱含量的方法。方法 高效液相色谱法测定生物碱,色谱条件:色谱柱,Wondasil C18(4.6mm×250mm,5μm);流动相,乙腈-0.05mol/L磷酸二氢钾水溶液(三乙胺2%,15:85,v/v);流速,1.0ml/min,检测波长:205nm。结果 苦参碱、氧化苦参碱、槐定碱和槐果碱分别在0.076~0.5,0.084~0.4,0.12~0.44,0.20~0.49μg/ml浓度范围内呈良好线性关系(R2>0.998),含量分别为:0.70%,0.28%,0.60%,0.01%。结论 本实验建立的HPLC法能快速同时测定苦参碱杀虫剂中4种生物碱的含量,可用于快速测定药品或环境中的生物碱。 |
英文摘要: |
Objective To establish a method for the simultaneous determination of the content of matrine,oxymatrine,sophoridine and sophocarpine in matrine pesticides by HPLC.Methods The alkaloids was determined by HPLC.The chromatographic conditions were established with WondasilC18 (4.6mm×250mm,5μm),mobile phase of acetonitrile-0.05mol/L potassium dihydrogen phosphate (2% triethylamine,15:85,v/v),flow rate of 1.0 mL/min and detection wavelength of 205nm.Results The matrine,oxymatrine,sophoridine and sophocarpine has good linearity relation (R2>0.999) at the range of 0.076~0.50、0.084~0.47、0.12~0.44、0.20~0.49μg/mL,respectively.The contents were 0.70%,0.28%,0.60%,and 0.01%,respectively.Conclusion The four alkaloids were determined simultaneously by HPLC method in matrine pesticides,which can be used to determine the alkaloids content in drug or environment. |
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