任强,薛永,聂其婷,王亚楠,夏天睿.紫外分光光度法测定不同产地半枝莲总黄酮含量[J].济宁医学院学报,2017,40(2):103-105 |
紫外分光光度法测定不同产地半枝莲总黄酮含量 |
Determination of total flavonoids from different production areas of Scutellaria barbata D.Don by UV Spectrophotometry |
投稿时间:2016-11-19 |
DOI:10.3969/j.issn.1000-9760.2017.02.006 |
中文关键词: 半枝莲;总黄酮;紫外分光光度法;含量测定 |
英文关键词: Scutellaria barbata;Flavonoids;UV spectrophotometry;Determination |
基金项目:山东省医药卫生科技发展计划项目(2014WS0508);济宁医学院大学生创新创业训练计划资助项目(cx2015060) |
作者 | 单位 | 任强 | 济宁医学院药学院, 日照 276826 | 薛永 | 济宁医学院药学院, 日照 276826 | 聂其婷 | 济宁医学院药学院, 日照 276826 | 王亚楠 | 济宁医学院药学院, 日照 276826 | 夏天睿 | 广东一方制药有限公司, 佛山 528244 |
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中文摘要: |
目的 建立紫外分光光度法,测定不同产地半枝莲中的总黄酮含量。方法 采用紫外分光光度法,以黄芩苷为对照品,对15个不同产地半枝莲中的总黄酮进行含量测定。结果 紫外分光光度法测定黄芩苷,在2.4~12μg/ml范围内呈良好的线性关系C=0.01593×(Abs)+0.00016 (r=0.9997),不同产地的15个半枝莲样品进行测定,总黄酮含量分别为2.70%、1.85%、2.18%、2.36%、2.29%、2.30%、1.64%、1.99%、2.42%、2.22%、0.97%、1.99%、1.44%、2.11%、2.56%。结论 不同产地半枝莲中的总黄酮含量差异明显,其中编号1产地含量最高,编号11地区含量最低。 |
英文摘要: |
Objective Establish a UV spectrophotometric method,determination of total flavonoids in Scutellaria barbata D.Don from different regions.Methods A total of flavonoids in Scutellaria barbata D.Don from 15 different places were determined by means of ultraviolet spectrophotometry,with baicalin as reference substance.Results Determination of baicalin by UV Spectrophotometry;the calibration curve C=0.015933×(Abs)+0.00016 was linear in the range of 2.4μg/ml~12μg/ml with good linearity (r=0.9997),the total flavonoids content from different regions were 2.70%,1.85%,2.18%,2.36%,2.29%,2.30%,1.64%,1.99%,2.42%,2.22%,0.97%,1.99%,1.44%,2.11%,2.56%.Conclusion The different samples had obvious difference of the content,which No.1 sample was highest No.11 sample content was the lowest. |
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