张兆强,张鲁华,林立,张春芝.矽尘对实验小鼠肺组织TOLL样受体2、4、9 mRNA表达的影响[J].济宁医学院学报,2017,40(1):21-24 |
矽尘对实验小鼠肺组织TOLL样受体2、4、9 mRNA表达的影响 |
Effects of silica dust on expression of TLR2,4,9 mRNA in lung tissue of mice |
投稿时间:2016-08-09 |
DOI:10.3969/j.issn.1000-9760.2017.01.005 |
中文关键词: 矽尘;肺纤维化;TLR2;TLR4;TLR9 |
英文关键词: Silica dust;Lung fibrosis;TLR2;TLR4;TLR9 |
基金项目:山东省卫生与计划生育委员会(2014WS0510);济宁医学院科研计划项目(JY2015KJ003) |
作者 | 单位 | 张兆强 | 济宁医学院公共卫生学院, 济宁 272067 | 张鲁华 | 济宁医学院附属济宁市第一人民医院, 济宁 272011 | 林立 | 济宁医学院公共卫生学院, 济宁 272067 | 张春芝 | 济宁医学院公共卫生学院, 济宁 272067 |
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中文摘要: |
目的 探讨矽尘对实验小鼠肺组织TOLL样受体2、4、9(TLR2、4、9)mRNA表达的影响。方法 将40只小鼠随机分成对照组(不染尘)、2h组(每天染尘2 h)、4h组(每天染尘4 h)和8h组(每天染尘8 h),每组10只。20 d后,处死动物,取肺组织,提取总RNA、实时荧光定量PCR(RT-qPCR)检测不同暴露时间组TLR2、4、9 mRNA的相对含量。结果 对照组与3个暴露时间组TLR2 mRNA 的相对含量分别为:(0.98±0.69)、(1.18±0.85)、(2.56±0.54)、(3.22±0.77),4组间的差异有统计学意义(F=13.70,P=0.00);与对照组相比,4h、8h组的表达升高,差异有统计学意义(P< 0.05)。对照组与3个暴露时间组TLR4 mRNA 的相对含量分别为:(0.93±0.32)、(1.16±1.23)、(4.80±5.31)、(7.00±6.22),4组间的差异有统计学意义(F=5.06,P=0.00);与对照组相比,4h、8h组TLR4 mRNA的表达升高,差异统计学意义(P< 0.05)。对照组与3个暴露时间组TLR9 mRNA 的相对含量分别为:(1.04±0.25)、(1.29±0.30)、(1.26±0.41)、(2.21±0.33),4组间的差异有统计学意义(F=15.82,P=0.00);与对照组相比,仅8h组的TLR9 mRNA 表达升高,差异有统计学意义(P< 0.05)。结论 染矽尘后,小鼠肺组织TLR2、4、9 mRNA的表达上调,这可能是启动肺纤维化的信号转导的起始步骤。 |
英文摘要: |
Objective To study the expression of TLR2,4,9 mRNA of lung in mice exposed to silica dust.Methods 40 mice were randomly divided into 4 groups including control group,2h group (exposed to silica dust for 2 h every day),4h group (exposed to silica dust for 4 h every day) and 8h group (exposed to silica dust for 8 h every day).20 days later,all the lung of mice were taken to measure the expression of TLR2,4,9 mRNA by Realtime fluorescence quantitative PCR (RT-qPCR) technology.Results The relative contents of TLR2 mRNA of the control,2h,4h and 8h groups were (0.98±0.69),(1.18±0.85),(2.56±0.54) and (3.22±0.77).There were significantly difference among them (F=13.70,P=0.00).Compared with the control group,the expression of TLR2 mRNA of the 4h and 8h groups were significantly higher (P< 0.05).The relative contents of TLR4 mRNA of the control,2h,4h and 8h groups were (0.93±0.32),(1.16±1.23),(4.80±5.31) and (7.00±6.22).There were significantly difference among the 4 groups (F=5.06,P=0.00).Compared with the control group,the expression of TLR4 mRNA in the 4h and 8h groups were higher (P< 0.05).The relative contents of TLR9 mRNA of the control,2h,4h and 8h groups were (1.04±0.25),(1.29±0.30),(1.26±0.41) and (2.21±0.33).There were significantly difference among them (F=15.82,P=0.00).Compared with the control group,only the expression of TLR9 mRNA of the 8h groups were higher (P< 0.05).Conclusion The expression of TLR2,4,9 mRNA of the lung tissue of mice exposed to silica dust were up-regulated,which might be the initial step of the signal pathway leading to lung fibrosis. |
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