王一宇,孙盼盼,史艳芬,宋静.复方氯己定对口腔种植体周围炎病原菌生物膜的影响[J].济宁医学院学报,2016,39(6):395-398 |
复方氯己定对口腔种植体周围炎病原菌生物膜的影响 |
Effect of compound chlorhexidine on the biofilm of the pathogen of oral peri-implantitis |
投稿时间:2016-05-04 |
DOI:10.3969/j.issn.1000-9760.2016.06.004 |
中文关键词: 复方氯己定;牙龈卟啉单胞菌;生物膜 |
英文关键词: Compound chlorhexidine;Porphyromonas gingivalis;Biofilm |
基金项目: |
作者 | 单位 | 王一宇 | 济宁医学院口腔学院, 济宁 272067 | 孙盼盼 | 济宁医学院口腔学院, 济宁 272067 | 史艳芬 | 济宁医学院口腔学院, 济宁 272067 | 宋静 | 济宁医学院, 济宁 272067 |
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中文摘要: |
目的 探讨复方氯己定对种植体周围炎病原菌牙龈卟啉单胞菌菌斑生物膜的影响。方法 在钛片上建立牙龈卟啉单胞菌菌斑生物膜,通过扫描电镜观测其生长状况以及加入复方氯己定后的生长状况;激光共聚焦显微镜观察加入复方氯己定对生物膜膜层厚度的影响;采用结晶紫法检测复方氯己定对牙龈卟啉单胞菌菌斑生物膜的影响。结果 扫描电镜显示加入复方氯己定后种植体周围炎病原菌未能形成良好的生物膜,激光共聚焦显示加入复方氯己定后生物膜整体为红色荧光,说明出现了较多死菌,氯己定破坏了生物膜的完整性;结晶紫法测得复方氯己定组OD值(0.206±0.007),与阴性对照组(0.351±0.005)相比,显著下降,差异具有统计学意义(P<0.05)。结论 作为口腔常见含漱液之一的复方氯己定对牙龈卟啉单胞菌菌菌斑生物膜的形成有一定程度的抑制作用。 |
英文摘要: |
Objective To study the effect of compound chlorhexidine on the biofilm of the pathogen of oral peri-implantitis.Methods We established plaque biofilm of the pathogen on the surface of titanium and observed the growth condition between and after adding compound chlorhexidine by scanning electron microscope (SEM).The effects of compound chlorhexidine on the biofilm of Porphyromonas gingivalis were investigated using crystal violet method.CLSM was used to observe activity of compound chlorhexidine on bacterial biofilm.SPSS13.0 was used for statistical analysis.Results SEM observation in the experimental group added compound chlorhexidine showed the failure to form a good biofilm.The crystal violet assay showed that compound chlorhexidine had the inhibitory effect on plaque biofilms (P<0.05).CLSM showed that biofilm thickness was decreased after adding compound chlorhexidine (P<0.05).Conclusion As one of the common oral gargle,compound chlorhexidine has a certain inhibition on the formation of Porphyromonas gingivalis bacteria biofilm. |
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