秦燕霞,李京,柳新平.siRNA沉默B7-H4基因对人卵巢癌细胞增殖能力的影响[J].济宁医学院学报,2016,39(5):320-323 |
siRNA沉默B7-H4基因对人卵巢癌细胞增殖能力的影响 |
Effect of silencing B7-H4 expression by small interference RNA on proliferation of ovarian carcinoma cells |
投稿时间:2016-04-15 |
DOI:10.3969/j.issn.1000-9760.2016.05.005 |
中文关键词: 卵巢癌;B7-H4;siRNA;增殖 |
英文关键词: Ovarian carcinoma;B7-H4;siRNA;Proliferation |
基金项目:2015年济宁医学院校级科研项目(JY2015KJ018) |
作者 | 单位 | 秦燕霞 | 济宁医学院基础医学院, 济宁 272067 | 李京 | 济宁医学院基础医学院, 济宁 272067 | 柳新平 | 济宁医学院基础医学院, 济宁 272067 |
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中文摘要: |
目的 探讨B7-H4基因的小干扰RNA(small interference RNA,siRNA)对卵巢癌SKOV-3细胞生长及增殖的影响。方法 化学合成B7-H4-siRNA,转染SKOV-3细胞,MTT法检测卵巢癌细胞增殖情况,细胞计数法计算细胞倍增时间。结果 B7-H4-siRNA成功转入SKOV-3细胞,B7-H4-siRNA组细胞生长受到明显抑制,细胞倍增时间明显长于未转染组、随机乱码组和转染试剂对照组。结论 B7-H4-siRNA能沉默SKOV-3细胞中B7-H4的表达,抑制SKOV-3细胞的生长增殖,B7-H4可能成为卵巢癌基因治疗的候选靶点。 |
英文摘要: |
Objective To investigate the effect of silencing B7-H4 expression by small interference RNA (siRNA) on proliferation of ovarian carcinoma cells.Methods Chemical synthesized siRNA targeting B7-H4 was transfected into SKOV-3 cells.The proliferation of SKOV-3 cells was determined by MTT assay and cell doubling time was detected by cell counting method.Results B7-H4-siRNA was successfully transfected into SKOV-3 cells.In the B7-H4-siRNA group,the proliferation of SKOV-3 cells was significantly inhibited,and the doubling time was longer than that in the untransfected group,the Ctrl-siRNA group and the transfection reagent group.Conclusion B7-H4-siRNA can silence B7-H4 expression in SKOV-3 cells,and inhibit the proliferation of SKOV-3 cells.B7-H4 can be regarded as a candidate gene for ovarian carcinoma gene therapy. |
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