| 王琳,刘同美,张代娟,刘江月.青心酮对兔动脉粥样硬化血管细胞黏附因子-1蛋白表达的影响[J].济宁医学院学报,2013,(2):100-102,105 |
| 青心酮对兔动脉粥样硬化血管细胞黏附因子-1蛋白表达的影响 |
| The effect of DHAP on the expression of VCAM-1 in atherosclerotic rabbits |
| 投稿时间：2013-02-11 |
| DOI：10.3969/j.issn.1000-9760.2013.02.007 |
| 中文关键词: 青心酮;血管细胞黏附因子-1;动脉粥样硬化 |
| 英文关键词: 3,4-dihydroxyacetophenone;Vascular cell adhesion molecule-1;Atherosclerosis |
| 基金项目: |
| 作者 | 单位 | E-mail | | 王琳 | 潍坊医学院病理生理教研室, 山东潍坊 261053 | | | 刘同美 | 潍坊医学院病理生理教研室, 山东潍坊 261053 | tongmeiliu@126.com | | 张代娟 | 潍坊医学院病理生理教研室, 山东潍坊 261053 | | | 刘江月 | 潍坊医学院病理生理教研室, 山东潍坊 261053 | |
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| 中文摘要: |
| 目的 探讨新西兰大白兔动脉粥样硬化病变中血管细胞黏附因子-1(VCAM-1)的表达和青心酮抗动脉粥样硬化作用。方法 18只新西兰大耳白兔随机分为常规饲料组、胆固醇饲料组和青心酮组,饲养12周。实验前后检测血清胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL),采用免疫组化和Western blot法测定主动脉VCAM-1阳性表达百分比和蛋白的表达。结果 青心酮显著降低TC、TG和LDL水平(P<0.05)。胆固醇组和青心酮组病变局部VCAM-1的阳性细胞百分比和蛋白的表达含量明显高于常规饲料组(P<0.05)。但青心酮组动脉粥样硬化改变较胆固醇组明显减轻,VCAM-1阳性百分比和蛋白的表达量较胆固醇饲料组明显降低(P<0.05)。结论 动脉粥样硬化的发生伴有血管细胞黏附因子的高度表达,青心酮不但有调脂作用,且具有抑制黏附分子表达和抗动脉粥样硬化的作用。 |
| 英文摘要: |
| Objective To explore the effect of DHAP on the expression of VCAM-1 in atherosclerotic rabbits.Methods 18 rabbits were randomly divided into three groups:normal group,cholesterol diet group,DHAP treatment group.All rabbits were fed for 12 weeks.Serum TC,TG,LDL were measured in 0 and 12 weeks in three groups.At 12 week,we detected the aortic VCAM-1 positive percentage by immunohistochemistry assay and the expression of VCAM-1 protein by Western blot.Results DHAP can obviously decrease the level of TC TG and LDL(P<0.05).The measurement of VCAM-1 positive percentage and the expression of VCAM-1 protein both in cholesterol diet group and DHAP treatment group were higher than normal group(P<0.05),but pathologic changes in DHAP treatment group were lower than cholesterol group(P<0.05).The measurement of VCAM-1 positive percentage and the expression of VCAM-1 protein were also lower than cholesterol diet group(P<0.05).Conclusion VCAM-1 can be highly expressed in atherosclerosis.DHAP can not only decrease cholesterol but also inhibit expression of VCAM-1 which has the effect of anti-atherosclerosis. |
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