| 梁啸,刘洪美,李庆伟,陈国武,孟纯阳.对SNI和CCI两种大鼠神经病理性疼痛模型的实验观察[J].济宁医学院学报,2013,(1):22-24,27 |
| 对SNI和CCI两种大鼠神经病理性疼痛模型的实验观察 |
| Experimental observation of SNI and CCI two kinds of rat neuropathic pain models |
| 投稿时间：2012-11-11 |
| DOI：10.3969/j.issn.1000-9760.2013.01.005 |
| 中文关键词: 神经病理性疼痛;大鼠;动物模型;GCH1 |
| 英文关键词: Neuropathic pain;Rat;Animal model;GCH1 |
| 基金项目:山东省教育厅高校科技计划资助课题(J11LF19) |
| 作者 | 单位 | E-mail | | 梁啸 | 新乡医学院研究生处, 河南新乡 453000 | | | 刘洪美 | 济宁医学院基础学院, 山东济宁 272067 | | | 李庆伟 | 济宁医学院附属医院, 山东济宁 272029 | | | 陈国武 | 济宁医学院附属医院, 山东济宁 272029 | | | 孟纯阳 | 济宁医学院附属医院, 山东济宁 272029 | chunyangmeng16@163.com |
|
| 摘要点击次数: 6015 |
| 全文下载次数: 1148 |
| 中文摘要: |
| 目的 寻找无创、直接有效的靶向中枢给药方法,将降钙素基因相关肽(CGRP)避开血脑屏障(BBB)直接运送到中枢神经系统。方法 大鼠采取线栓法制作大脑中动脉闭塞模型(MCAO),在随机双盲状态下分组予以不同处理。利用酶联免疫吸附法(ELISA)测定经鼻腔和静脉注射CGRP后30min时,各脑区的CGRP浓度,研究CGRP经鼻给药的可行性。结果 IN组脑部各区域、颈髓和CSF中CGRP浓度较IV组显著增高(P<0.01),以嗅球的CGRP浓度最高,其次为CSF和颈髓。结论 经鼻靶向中枢给药可以避开血脑屏障的阻碍,将CGRP递送至中枢神经系统,中枢神经系统的CGRP浓度显著提高。 |
| 英文摘要: |
| Objective To observe and compare the efficacy of 2 neuropathic pain models:spared nerve injury model (SNI) and chronic constriction injury of sciatic nerve model (CCI).Methods 30 Wistar rats were divided randomly into 3 groups:sham group (n=10),SNI group (n=10),and CCI group (n=10).In the sham group,the sciatic nerves of rats were just surgically exposed,and the SNI model and CCI model were operated on the right posterior limb.The mechanical withdrawal threshold (MWT) were tested from the 1th day preoperative to the 28th day postoperative,and GTP cyclohydrolase 1 (GCH1),which is a kind of pain-related gene,in dorsal root ganglion was measured by Western blotting on the 14th and 28th days postoperative.Results The rats in sham group did not appear hyperalgia.MWT in both SNI group and CCI group were obviously declined on the 1th day postoperative compared with Sham group (P<0.05).MWT of SNI rats keeped a low level to the 28th day postoperative,but MWT in CCI group rose again from the 21th day postoperative.The results of Western blotting showed that,on the 14th day after the operation,the expression of GCH1 in both SNI and CCI group increased compared with sham group (P<0.05),but on the 28th day it decreased in CCI group,and there were statistical differences compared with SNI group (P<0.05).Conclusion Compared with CCI model,SNI model is a more sensitive and stable neuropathic pain model.Key words:Neuropathic pain; Rat; Animal model; GCH1 |
|
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
