| 周英泽,王桂芬,刘江月,张代娟,刘同美.A30Pα-synuclein原核表达载体的构建及表达[J].济宁医学院学报,2012,(2):97-99 |
| A30Pα-synuclein原核表达载体的构建及表达 |
| Construction of prokaryotic expression vector of A30Pα-synuclein and expression in E.coli BL21 |
| 投稿时间：2012-04-01 |
| DOI：10.3969/j.issn.10009760.2012.02.004 |
| 中文关键词: A30Pα-synuclein;帕金森病;GST-融合蛋白 |
| 英文关键词: A30Pα-synuclein;Parkinson's disease;GST-fusion protein |
| 基金项目:山东省卫生厅青年基金资助项目(编号:2007QW007) |
| 作者 | 单位 | E-mail | | 周英泽 | 潍坊医学院病理生理学教研室, 山东潍坊 261053 | | | 王桂芬 | 潍坊医学院病理生理学教研室, 山东潍坊 261053 | | | 刘江月 | 潍坊医学院病理生理学教研室, 山东潍坊 261053 | | | 张代娟 | 潍坊医学院病理生理学教研室, 山东潍坊 261053 | | | 刘同美 | 潍坊医学院病理生理学教研室, 山东潍坊 261053 | blshl@wfmc.edu.cn |
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| 中文摘要: |
| 目的 构建A30Pα-synuclein基因的原核表达载体,分析其在大肠杆菌中的表达。方法 酶切质粒pcDNA3.0-A30Pα-synuclein,获得A30Pα-synuclein基因的目的片段,克隆至原核表达载体pGEX-6P-1中,转化大肠杆菌BL21。IPTG诱导后,经考马斯亮蓝染色和Western-blot 分析目的蛋白的表达。结果 A30Pα-synuclein基因克隆至pGEX-6P-1载体中,考马斯亮蓝染色及Western-blot检测到A30Pα-synuclein蛋白在BL21中的表达。结论 成功构建A30Pα-synuclein的原核表达载体,并在大肠杆菌中表达了A30Pα-synuclein融合蛋白,为进一步研究A30Pα-synuclein在帕金森病中的作用奠定了良好基础。 |
| 英文摘要: |
| Objective To construct a prokaryotic expression vector of A30P α-synuclein and analyze its expression in E.coli BL21. Methods The fragment of A30Pα-synuclein was cloned into pGEX-6P-1 vector correctly and then transformed into E.coli BL21. The expression of A30Pα-synuclein was induced with IPTG, and analyzed by Coomassie brilliant blue dyeing and Western-blot.Results A30P α-synuclein was cloned into pGEX-6P-1 properly. The recombinant fusion protein was expressed in E.coli BL21 by Coomassie brilliant blue dying and Western-blot.Conclusion The prokaryotic expression vector of A30P α-synuclein has been successfully constructed and expressed in E.coli BL21 which provides a foundation for the further study of A30Pα-synuclein in Parkinson's disease. |
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